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Mouse beta Defensin-3 Recombinant

BD-3

accession:  Q9WTL0
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2700
Domain  :  Defensin
Gene  :  DEFB103A
Catalog no. :  RKQ9WTL0
Source:
Optimized DNA sequence encoding Mouse beta Defensin-3 mature chain was expressed in Escherichia Coli.

 
Molecular weight:
Native Mouse BD-3 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 5 kDa.

Recombinant beta Defensin-3 is a disulfide-linked monomer protein consisting of 42 amino acid residue subunits, and migrates as an approximately 5 kDa protein under non-reducing and  reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was Determined by its ability to chemoattract immature human dendritic cells using a concentration range of 1-50 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MRIHYLLFAF LLVLLSPPAA FSKKINNPVS CLRKGGRCWN RCIGNTRQIG SCGVPFLKCC 


KRK 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Mouse beta Defensin-3 was lyophilized from 0.2 μm filtered 100mM NaCl, 20mM PB, pH 7.4.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

beta Defensin-3

Defensins are a large family of peptides of which two groups exist in mammals: alpha defensins and beta defensins, which are distinguishable by the spacing and connectivity of the conserved cysteine residues within the mature peptides.It is thought that defensins function in the eradication of pathogens from the host system by inserting themselves into the bacterial membrane under the influence of membrane potential, forming channels which lead to leakage of cytoplasmic molecules and cell death. Unlike hBD-1, this second peptide, human beta-defensin-2 (hBD-2), is regulated at a transcriptional level in response to contact with microorganisms, and is highly eåective in killing the gram negative bacteria Its expression is also upregulated by the cytokine tumor necrosis factor-alpha.

Related Publications:
persistent nasal carriage of staphylococcus aureus is associated with deficient induction of human β-defensin 3 after sterile wounding of healthy skin in vivo
Infect. Immun., Jul 2011; 79: 2658 - 2662.
human β-defensin 2 and β-defensin 3 chimeric peptides reveal the structural basis of the pathogen specificity of their parent molecules
Antimicrob. Agents Chemother., Mar 2011; 55: 954 - 960.
reduced human beta defensin 3 in individuals with periodontal disease
Journal of Dental Research, Feb 2011; 90: 241 - 245.
human β-defensin 3 promotes nf-b-mediated ccr7 expression and anti-apoptotic signals in squamous cell carcinoma of the head and neck
Carcinogenesis, Feb 2011; 32: 168 - 174.
severity of staphylococcus aureus infection of the skin is associated with inducibility of human β-defensin 3 but not human β-defensin 2
Infect. Immun., Jul 2010; 78: 3112 - 3117.




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product_icon_8.jpg Mouse beta Defensin-3 Recombinant
product_icon_8.jpg Human beta Defensin-3 Recombinant
 
 
 
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