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Mouse beta Defensin-2 Recombinant

BD-2

accession:  P82020
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2700
Domain  :  Defensin
Gene  :  DEFB4
Catalog no. :  RKP82020

 

Source:
Optimized DNA sequence encoding Human beta Defensin-2 mature chain was expressed in Escherichia Coli.

 
Molecular weight:
Native Mouse BD-2 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 6 kDa.

Recombinant Mouse beta Defensin-2 is a disulfide-linked monomer protein consisting of 52 amino acid residue subunits, and migrates as an approximately 6 kDa protein under non-reducing and  reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was Determined by its ability to chemoattract immature human dendritic cells using a concentration range of 1-50 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MRTLCSLLLI CCLLFSYTTP AVGSLKSIGY EAELDHCHTN GGYCVRAICP PSARRPGSCF 

        70 
PEKNPCCKYM K 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Mouse beta Defensin-2 was lyophilized from 0.2 μm filtered 100mM NaCl, 20mM PB, pH 7.4.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
 

 

beta Defensin-2

Defensins are a large family of peptides of which two groups exist in mammals: alpha defensins and beta defensins, which are distinguishable by the spacing and connectivity of the conserved cysteine residues within the mature peptides.It is thought that defensins function in the eradication of pathogens from the host system by inserting themselves into the bacterial membrane under the influence of membrane potential, forming channels which lead to leakage of cytoplasmic molecules and cell death. Unlike hBD-1, this second peptide, human beta-defensin-2 (hBD-2), is regulated at a transcriptional level in response to contact with microorganisms, and is highly eåective in killing the gram negative bacteria Its expression is also upregulated by the cytokine tumor necrosis factor-alpha.

Related Publications:
beta-defensin 2 promotes resistance against infection with p. aeruginosa
Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 848.
ccr6 regulation of the actin cytoskeleton orchestrates human beta Defensin-2- and ccl20-mediated restitution of colonic epithelial cells
J. Biol. Chem., Apr 2009; 284: 10034 - 10045.
β-defensin-2 promotes resistance against infection with p. aeruginosa
J. Immunol., Feb 2009; 182: 1609 - 1616.
direct screening identifies mature β-defensin 2 in avian heterophils
Poult. Sci., Feb 2009; 88: 372 - 379.
leptin enhances human β-defensin-2 production in human keratinocytes
Endocrinology, Oct 2008; 149: 5189 - 5198.




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Antibodies:
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product_icon_8.jpg Human beta Defensin-2 Recombinant
product_icon_8.jpg Mouse beta Defensin-2 Recombinant
 
 
 
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