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Rat Insulin Like Growth Factor-I Recombinant

IGF-I

accession:  P08025
   Size A     10ug   $ 70
   Size B      50ug  $160
   Size C      1mg  $ 900
Domain  :  Insulin
Gene  :  IGF1
Catalog no. :  RKP08025
Source:
Optimized DNA sequence encoding Rat Insulin Growth Factor -1mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native Rat Insulin Growth Factor-1 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 8 kDa.

 

Recombinant rat IGF-1 is a disulfide-linked monomeric protein consisting of 71 amino acid residue subunits. IGF-1 migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED50 was determined by a cell proliferation assay using FDC-P1 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MGKISSLPTQ LFKCCFCDFL KVKMHTMSSS HLFYLALCLL TFTSSATAGP ETLCGAELVD 

        70         80         90        100        110        120 
ALQFVCGPRG FYFNKPTGYG SSIRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPTKSARS 

       130        140        150 
VRAQRHTDMP KTQKEVHLKN ASRGSAGNKN YRM 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Rat IGF1 was lyophilized from a 0.2 μm filtered  PBS solution, pH 7.4.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

IGF-I

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I is synthesized as two precursor isoforms with N- and alternate C- terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C- terminal regions. Mature human IGF-I shares 94% and 96% aa sequence identity with mouse and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF-II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects.

Related Publications:
statement by the growth hormone research society on the gh/IGF-I axis in extending health span
J Gerontol A Biol Sci Med Sci, Oct 2009; 64A: 1039 - 1044.
partial primary deficiency of insulin-like growth factor (igf)-i activity associated with igf1 mutation demonstrates its critical role in growth and brain development
J. Clin. Endocrinol. Metab., Sep 2009; 10.1210/jc.2009-0452.
myostatin inhibits IGF-I induced myotube hypertrophy through akt
Am J Physiol Cell Physiol, Sep 2009; 10.1152/ajpcell.00043.2009.
the insulin-like growth factor (igf)-i e-peptides modulate cell entry of the mature IGF-I protein
Mol. Biol. Cell, Sep 2009; 20: 3810 - 3817.
elevated levels of insulin-like growth factor (igf)-i in serum rescue the severe growth retardation of IGF-I null mice
Endocrinology, Sep 2009; 150: 4395 - 4403.




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