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Rat LIX (CXCL5) Recombinant

CXCL5

accession:  P97885
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2700
Domain  : 
Gene  :  CXCL5
Catalog no. :  RKP97885
Source:
Optimized DNA sequence encoding rat LIX (CXCL5) mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Rat LIX , generated by the proteolytic removal of the signal peptide
and propeptide and has a calculated molecular mass of approximately 11 kDa.

Recombinant rat CXCL5 is a disulfide-linked monomeric protein consisting of 94 amino acid residue subunits and migrates as an approximately 11 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Determined by its ability to chemoattract human neutrophils using a concentration range of 2.0-40.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MSFQLRSSAR IPSRSCSSFT LLAFLLLFTL PQHRAQAAPF SAMVATELRC VCLTLAPRIN 

        70         80         90        100        110        120 
PKMIANLEVI PAGPHCPKVE VIAKLKNQKD NVCLDPQAPL IKKVIQKILG SENKKTKRNA 

       130 
LALVRSASTQ 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant CXCL5 was lyophilized from a 0.2 μm filtered 20mM PB,100mM NaCl solution pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

CXCL5

Epithelial neutrophil- activating protein 78 (ENA-78) is a member of the CXC chemokines and acts as a potent chemoattractant and activator of neutrophil function. On stimulation in vitro, ENA-78 is highly expressed in many cell types. ENA-78 protein levels are strongly elevated in synovial fluid and blood of patients with rheumatoid arthritis. By in situ hybridization and immunofluorescence staining, ENA-78 has been recognized as a major CXC chemokine expressed in epithelial cells of the intestinal mucosa of patients with Crohn’s disease, ulcerative colitis, and acute appendicitis.

Related Publications:
CXCL5 mediates uvb irradiation–induced pain
Science Translational Medicine, Jul 2011; 3: 90ra60.
il-17a and tnf- exert synergistic effects on expression of CXCL5 by alveolar type ii cells in vivo and in vitro
J. Immunol., Mar 2011; 186: 3197 - 3205.
evaluation of serum CXCL5 as a serum marker for prognosis of colorectal cancer patients.
ASCO Meeting Abstracts, Feb 2011; 29: 442.
cxcl1 regulates pulmonary host defense to klebsiella infection via cxcl2, CXCL5, nf-b, and mapks
J. Immunol., Nov 2010; 185: 6214 - 6225.
posttranslational modification of the nh2-terminal region of CXCL5 by proteases or peptidylarginine deiminases (pad) differently affects its biological activity
J. Biol. Chem., Sep 2010; 285: 29750 - 29759.




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Antibodies:
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product_icon_8.jpg Rat LIX (CXCL5) Recombinant
 
 
 
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