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Mouse MIP-3 beta (CCL19) Recombinant

MIP3

accession:  O70460
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2700
Domain  :  IL8
Gene  :  CCL19
Catalog no. :  RKO70460
Source:
Optimized DNA sequence encoding Mouse MIP-3 (CCL19) mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Mouse CCL19, generated by the proteolytic removal of the signal peptide
and propeptide and has a calculated molecular mass of approximately 10kDa.

Recombinant MIP-3b(CCL19) is a disulfide-linked monomeric protein consisting of 84 amino acid residue subunits and migrates as an approximately 11 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Determined by its ability to chemoattract human dendritic cells using a concentration range of 10.0-50.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MAPRVTPLLA FSLLVLWTFP APTLGGANDA EDCCLSVTQR PIPGNIVKAF RYLLNEDGCR 

        70         80         90        100        110 
VPAVVFTTLR GYQLCAPPDQ PWVDRIIRRL KKSSAKNKGN STRRSPVS 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant CCL19 was lyophilized from a 0.2 μm filtered 20mM PB,100mM NaCl solution pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

CCL19

The chemokine MIP-3β is chemoattractant for T and B lymphocytes, dendritic cells , macrophage progenitor cells , and natural killer cells. It might, therefore, play an important role in the trafficking of T cells in the thymus and migration of T and B cells to secondary lymphoid organs . Furthermore, MIP-3β has been recently shown to mediate rapid adhesion of naive CD4+ T lymphocytes to activated endothelial cells supporting the role of this chemokine in regulation of lymphocyte homing. MIP-3β acts through CC chemokine receptor 7 (CCR7). The chemokines Lkn-1 and MIP-3α are also lymphocyte attractants, although compared with MIP- 3β, their promigratory effect on lymphocytes is less intensive and their chemotactic activity is more promiscuous. Lkn-1 attracts monocytes, lymphocytes, and eosinophils via the chemokine receptors CCR1 and CCR3. MIP-3α exhibits promigratory effects on lymphocytes, neutrophils, and immature dendritic cells via the CCR6 receptor.

Related Publications:
both dock180 and dock2 participate in the cxcl12 and CCL19 dependent chemotaxis of human dendritic cells derived from monocytes through the activation of rac
Blood (ASH Annual Meeting Abstracts), Nov 2011; 118: 521.
CCL19 induced responses are differentially regulated by atypical chemokine receptor cram and its classical chemokine receptor ccr7 in b-cll cells
Blood (ASH Annual Meeting Abstracts), Nov 2011; 118: 4896.
seminal fluid regulates accumulation of foxp3+ regulatory t cells in the preimplantation mouse uterus through expanding the foxp3+ cell pool and CCL19-mediated recruitment
Biol Reprod, Aug 2011; 85: 397 - 408.
dendritic cell chemotaxis in 3d under defined chemokine gradients reveals differential response to ligands ccl21 and CCL19
PNAS, Apr 2011; 108: 5614 - 5619.
pge2 transiently enhances dc expression of ccr7 but inhibits the ability of dcs to produce CCL19 and attract naive t cells
Blood, Sep 2010; 116: 1454 - 1459.




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