/, CCL chemokines/Mouse MIP-3 beta (CCL19) Recombinant

Mouse MIP-3 beta (CCL19) Recombinant

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$70.00$2,700.00

SKU: RKO70460 Tags: , , ,

Description

Accession
O70460
Source
Optimized DNA sequence encoding Mouse MIP-3 (CCL19) mature chain was expressed in Escherichia Coli.
Molecular weight
Mouse CCL19, generated by the proteolytic removal of the signal peptide and propeptide and has a calculated molecular mass of approximately10kDa. Recombinant MIP-3b(CCL19) is a monomeric protein consisting of amino acid residue subunits and migrates as an approximately11 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to chemoattract human dendritic cells using a concentration range of.0-50.0 ng/ml.

Protein Sequence
MAPRVTPLLA FSLLVLWTFP APTLGGANDA EDCCLSVTQR PIPGNIVKAF RYLLNEDGCR VPAVVFTTLR GYQLCAPPDQ PWVDRIIRRL KKSSAKNKGN STRRSPVS
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant CCL19 was lyophilized from a.2 μm filteredmM PB,100mM NaCl solution pH.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Q09163
Biological Process
Biological Process
Molecular function

Methods

Analysis of chemokine receptors and chemotaxis.

  • The migration of MSCs to different concentrations of CXCL12 and CCL19 was evaluated by chemotaxis assay.

Differential activation of MAP kinases in O. tsutsugamushi-infected DCs upon exposure to CCL19.

  • DCs were stimulated with O. tsutsugamushi , or LPS for 18 h and then further incubated with CCL19 (200 ng/ml) for the indicated times.

Effect of hematopoietic S1P lyase deficiency on T-cell proliferation and migration.

  • *P<0.05, **P<0.01, ***P<0.001 (WT versus KO), ###P<0.001 (PBS versus CCL19).

CyaA accelerates cell detachment and migration of TLR-activated DCs.

  • Migration of DCs treated with toxins and LPS (for 24 h) towards CCL19 or CCL21 (both 200 ng/ml) in transwell plates was determined by flow cytometry after additional 14 h (MDDCs) or 4 h (BMDCs) of incubation at 37°C.

Mst1 is required for integrin-independent T cell polarization.

  • A) Wt and Mst1 CD4 T cells were stimulated with 100 ng/mL CCL19 in PBS for 30 minutes.
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