Mouse MCP-5 (CCL12) Recombinant
Categories: CCL chemokinesRecombinant Human Cytokines$70.00 – $2,700.00
Description
Accession
Q62401
Source
Optimized DNA sequence encoding Mouse MCP-5(CCL12) mature chain was expressed in Escherichia Coli.
Molecular weight
Mouse MCP-5/CCL12, generated by the proteolytic removal of the signal peptide and propeptide and has a calculated molecular mass of approximately 10 kDa. Recombinant MCP-5/CCL12 is a monomer protein consisting of 83 amino acid residue subunits and migrates as an approximately 10 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to chemoattract human monocytes using a concentration range of.0-40.0 ng/ml.
Protein Sequence
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant MCP-5 was lyophilized from a 0.2 μm filteredmM PB solution pH.5,mM NaCl.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Molecular function
