///Mouse MIP-1gamma (CCL9) Recombinant

Mouse MIP-1gamma (CCL9) Recombinant

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$70.00$2,700.00

SKU: RKP51670 Tags: , , , , , , , , , ,

Description

Accession
P51670
Source
Optimized DNA sequence encoding mouse MIP-1 gamma chain was expressed in Escherichia Coli.
Molecular weight
Native mouse MIP-1gamma (CCL9) is generated by the proteolytic removal of the signal peptide and propeptide. This molecule has a calculated molecular mass of approximately 12 kDa. Recombinant CCL9 is a monomer protein consisting of 101 amino acid residue subunits,and migrates as an approximately 12 kDa protein under non-reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to chemoattract human neutrophiles using a concentration range of.0-10.0 ng/ml.

Protein Sequence
MKPFHTALSF LILTTALGIW AQITHATETK EVQSSLKAQQ GLEIEMFHMG FQDSSDCCLS YNSRIQCSRF IGYFPTSGGC TRPGIIFISK RGFQVCANPS DRRVQRCIER LEQNSQPRTY KQ
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant CCL9 was lyophilized from a.2 μm filtered PBS solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Q09163
Biological Process
Molecular function

Methods

Ccr1 ligands are induced after Candida infection and are chemotactic for kidney neutrophils ex vivo.

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