Description
Accession
P51670
Source
Optimized DNA sequence encoding mouse MIP-1 gamma chain was expressed in Escherichia Coli.
Molecular weight
Native mouse MIP-1gamma (CCL9) is generated by the proteolytic removal of the signal peptide and propeptide. This molecule has a calculated molecular mass of approximately 12 kDa. Recombinant CCL9 is a monomer protein consisting of 101 amino acid residue subunits,and migrates as an approximately 12 kDa protein under non-reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to chemoattract human neutrophiles using a concentration range of.0-10.0 ng/ml.
Protein Sequence
MKPFHTALSF LILTTALGIW AQITHATETK EVQSSLKAQQ GLEIEMFHMG FQDSSDCCLS YNSRIQCSRF IGYFPTSGGC TRPGIIFISK RGFQVCANPS DRRVQRCIER LEQNSQPRTY KQ
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant CCL9 was lyophilized from a.2 μm filtered PBS solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Q09163
Biological Process
Molecular function