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Mouse MCP-2 (CCL8) Recombinant

MCP-2

accession:  Q9Z121
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2700
Domain  :  IL8
Gene  :  MCP2
Catalog no. :  RKQ9Z121

(*)Complete precursor sequence shown, expressed chain highlighted
 

Source:
Optimized DNA sequence encoding mouse MCP-2(CCL8) mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Mouse MCP-2, generated by the proteolytic removal of the signal peptide
and propeptide and has a calculated molecular mass of approximately 9 kDa.

Recombinant MCP-2 is a disulfide-linked monomeric protein consisting of  79 amino acid residue subunits and migrates as an approximately 9 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent chemo-attraction human PBMC using a range of 10-100 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MKIYAVLLCL LLIAVPVSPE KLTGPDKAPV TCCFHVLKLK IPLRVLKSYE RINNIQCPME 

        70         80         90 
AVVFQTKQGM SLCVDPTQKW VSEYMEILDQ KSQILQP 
(*)Complete precursor sequence shown, expressed chain highlighted
 

Source:
Optimized DNA sequence encoding mouse MCP-2(CCL8) mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Mouse MCP-2, generated by the proteolytic removal of the signal peptide
and propeptide and has a calculated molecular mass of approximately 9 kDa.

Recombinant MCP-2 is a disulfide-linked monomeric protein consisting of  79 amino acid residue subunits and migrates as an approximately 9 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent chemo-attraction human PBMC using a range of 10-100 ng/ml.

 
Protein Sequence:

Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Mouse MCP-2 was lyophilized from a 0.2 μm filtered 20mM PB solution pH 7.5, 100mM NaCl.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

MCP-2

Monocyte chemotactic protein MCP-2 is a C-C chemokine.It shares over 60% amino acid identity with MCP-1 and MCP- 3 and has about 30% identity with other C-C chemokines MIP-1 alpha, RANTES, and MIP-1 beta.MCP-2 is chemotactic for and activates a wide variety of inflammatory cells, and its spectrum of action on leukocytes is similar to that of MCP-3, including monocytes, T lymphocytes, NK cells, basophils, mast cells, and eosinophils, but differs from MCP-1, which is not active on eosinophils. MCP-2 has been proposed to interact with multiple C-C chemokine receptors, including those used by MCP- 1 and MCP-3. However, since leukocytes express a multiplicity of chemokine receptors with promiscuous binding and functional properties, it is difficult to identify the receptors used by a given ligand on these cells.

Related Publications:
activated v9v2 t cells trigger granulocyte functions via MCP-2 release
J. Immunol., Jan 2009; 182: 522 - 529.
evaluating the potential of ip-10 and MCP-2 as biomarkers for the diagnosis of tuberculosis
Eur. Respir. J., Dec 2008; 32: 1607 - 1615.
changes of gene expression profiles in macrophages stimulated by angiotensin ii — angiotensin ii induces MCP-2 through at1-receptor
Journal of Renin-Angiotensin-Aldosterone System, Mar 2007; 8: 45 - 50.
neutrophil gelatinase b potentiates interleukin-8 tenfold by aminoterminal processing, whereas it degrades ctap-iii, pf-4, and gro- and leaves rantes and MCP-2 intact
Blood, Oct 2000; 96: 2673 - 2681.
expression of monocyte chemotactic protein (mcp)-1, MCP-2, and mcp-3 by human airway smooth-muscle cells . modulation by corticosteroids and t-helper 2 cytokines
Am. J. Respir. Cell Mol. Biol., Oct 1999; 21: 528.




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product_icon_8.jpg Human MCP-2 (CCL8) Recombinant
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