Description
Accession
Q8BSU2
Source
Optimized DNA sequence encoding mouse SR-PSOX (CXCL16) mature chain was expressed in Escherichia Coli.
Molecular weight
mouse CXCL16 , generated by the proteolytic removal of the signal peptide and propeptide and has a calculated molecular mass of approximately 10 kDa. Recombinant mouse CXCL16 is a monomeric protein consisting of 88 amino acid residue subunits and migrates as an approximately 10 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to chemoattract mouse lymphocytes using a concentration range of.0-40.0 ng/ml.
Protein Sequence
MRRGFGPLSL AFFLFLLALL TLPGDGNQGS VAGSCSCDRT ISSGTQIPQG TLDHIRKYLK AFHRCPFFIR FQLQSKSVCG GSQDQWVREL VDCFERKECG TGHGKSFHHQ KHLPQASTQT PEAAEGTPSD TSTPAHSQST QHSTLPSGAL SLNKEHTQPW EMTTLPSGYG LEARPEAEAN EKQQDDRQQE APGAGASTPA WVPVLSLLAI VFFLTAAMAY VLCNRRATQQ NSAGLQLWYT PVEPRP
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant CXCL16 was lyophilized from a 0.2 μm filteredmM PB,100mM NaCl solution pH.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
Methods
Transendothelial migration of mMSCs in response to chemokines.
- MAECs were treated with or without TNFα and in the presence or absence of CXCL16, CXCL9, CCL20 and CCL25 (each at 10 ng/ml).
