///Mouse PF4 (CXCL4) Recombinant

Mouse PF4 (CXCL4) Recombinant

Category:

$70.00$2,700.00

SKU: RKQ9Z126 Tags: , , , , ,

Description

Accession
Q9Z126
Source
Optimized DNA sequence encoding mouse PF4 CXCL4 mature chain was expressed in Escherichia Coli.
Molecular weight
Native mouse PF4 is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 8 kDa. Recombinant PF4 is a monomer protein consisting of 76 amino acid residue subunits, and migrates as an approximately kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to inhibit human FGF-basic-dependent proliferation of NR6R-3T3 mouse fibroblasts. The ED50 for this effect is typically-15 μg/mL.
Protein Sequence
MSVAAVFRGL RPSPELLLLG LLFLPAVVAV TSAGPEESDG DLSCVCVKTI SSGIHLKHIT SLEVIKAGRH CAVPQLIATL KNGRKICLDR QAPLYKKVIK KILES
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant mouse PF4/CXCL4 was lyophilized from a.2μm filtered concentrated (1mg/ml) solution inmM PB,1.5M NaCl pH.4.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Q2F862
Biological Process
Molecular function
Molecular function

Methods

Megakaryocytes control HSC quiescence via Cxcl4 (a) Left, gating strategy for sorting of Mk and immunofluorescence image of a Mk sorted accordingly.

  • (b) Bar graphs shows Cxcl4 mRNA levels in BM cell populations.
Stay in the loop
For new product information and regular updates on our product range please fill your details
Loading