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Mouse TECK (CCL25 ) Recombinant

TECK

Freeze transfer
accession:  O35903
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2700
Domain  :  IL8
Gene  :  CCL25
Catalog no. :  RKO35903
Source:
Optimized DNA sequence encoding mouse CCL25 mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native mouse CCL25 has a calculated molecular mass of approximately 14 kDa. 

Recombinant CCL25 is a disulfide-linked homodimeric protein consisting of 128 amino acid residue subunits, and migrates as an approximately 14 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>97%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Activity was determined by the ability to induce chemotaxis of purified human monocytes at concentrations ranging between 2-10 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MKLWLFACLV ACFVGAWMPV VHAQGAFEDC CLGYQHRIKW NVLRHARNYH QQEVSGSCNL 

        70         80         90        100        110        120 
RAVRFYFRQK VVCGNPEDMN VKRAIRILTA RKRLVHWKSA SDSQTERKKS NHMKSKVENP 

       130        140 
NSTSVRSATL GHPRMVMMPR KTNN 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant CCL25  was was lyophilized from  0.2μm filtered PBS , pH 7.4.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

CCL25

CCL25 is constitutively expressed in epithelium and mucosal vessels in the small bowel, where it interacts with gut-homing B and T cells expressing its receptor, CCR9.

Related Publications:
angiotensin-converting enzyme inhibition down-regulates the pro-atherogenic chemokine receptor 9 (ccr9)-chemokine ligand 25 (CCL25) axis
J. Biol. Chem., Jul 2010; 285: 23496 - 23505.
thymic progenitor homing and lymphocyte homeostasis are linked via s1p-controlled expression of thymic p-selectin/CCL25
J. Exp. Med., Apr 2009; 206: 761 - 778.
CCL25 increases thymopoiesis after androgen withdrawal
Blood, Oct 2008; 112: 3255 - 3263.
CCL25 and ccl28 promote 4β7-integrin-dependent adhesion of lymphocytes to madcam-1 under shear flow
Am J Physiol Gastrointest Liver Physiol, May 2008; 294: G1257 - G1267.
activation of ccr9/CCL25 in cutaneous melanoma mediates preferential metastasis to the small intestine
Clin. Cancer Res., Feb 2008; 14: 638 - 645.




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product_icon_8.jpg Human TECK (CCL25 ) Recombinant
product_icon_8.jpg Mouse TECK (CCL25 ) Recombinant
 
 
 
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