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Rat Ciliary Neurotrophic Factor Recombinant

CNTF

accession:  P20294
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2700
Domain  :  four helix cytokine core
Gene  :  CNTF
Catalog no. :  RKP20294
Source:
Optimized DNA sequence encoding Human Ciliary Neurotrophic Factor mature chain was expressed in Escherichia Coli.

 
Molecular weight:
Native human CNTF has a calculated molecular mass of approximately 22 kDa.

Recombinant CNTF  is a disulfide-linked monomer protein consisting of 200 amino acid residue subunits, and migrates as an approximately 22 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent proliferation of Human TF1 cells was found to be in the range of 2 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS 

        70         80         90        100        110        120 
TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA 

       130        140        150        160        170        180 
YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS 

       190        200 
SHQTGIPARG SHYIANNKKM 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Rat Ciliary Neurotrophic Factor was lyophilized from a 0.2 μm filtered PBS pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

CNTF

CNTF is found predominantly in peripheral nerve tissues. The main source appears to be myelin-associated Schwann cells in peripheral nerves and astrocytes in the central nervous system. CNTF is present at very high concentrations within intraocular tissues that contain the same muscle cells innervated by ciliary ganglionic neurons in vivo. CNTF receptors are expressed exclusively in the nervous system and skeletal muscle. The alpha subunit of the receptor is anchored to the cell membrane by a glycosyl- phosphatidylinositol linkage. The other receptor subunit, known as gp130, is also a component of receptors for LIF, Oncostatin M, IL6 and IL11, and seems to be involved also in signaling for CT-1. CNTF induces the expression of genes encoding hepatic acute phase proteins such as Haptoglobin, Alpha-1- antichymotrypsin, Alpha-2- Macroglobulin and Beta- fibrinogen in human hepatoma cells (HepG2) and in primary rat hepatocytes with a time course and dose-response comparable with that of IL6.

Related Publications:
the CNTF 1357 g >a polymorphism and the muscle strength response to resistance training.
J Appl Physiol, Jul 2009; 10.1152/japplphysiol.90835.2008.
humanin inhibits neuronal cell death by interacting with a cytokine receptor complex or complexes involving CNTF receptor /wsx-1/gp130
Mol. Biol. Cell, Jun 2009; 20: 2864 - 2873.
effect of CNTF on retinal ganglion cell survival in experimental glaucoma
Invest. Ophthalmol. Vis. Sci., May 2009; 50: 2194 - 2200.
CNTF over-expression leads to increased retinal ganglion cell survival in experimental glaucoma
Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 4052.
CNTF induces rapid expression of neuronal and glial transcripts in cultured porcine retinal progenitor cells
Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 1266.




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product_icon_8.jpg Human Ciliary Neurotrophic Factor Recombinant
product_icon_8.jpg Rat Ciliary Neurotrophic Factor Recombinant
 
 
 
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