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Mouse Keratinocyte Growth Factor Recombinant

KGF

accession:  P36363
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 4700
Domain  :  IL1
Gene  :  FGF7
Catalog no. :  RKP36363
Source:
Optimized DNA sequence encoding Mouse Keratinocyte Growth Factor mature chain was expressed in Escherichia Coli.

 
Molecular weight:
Native Mouse KGF is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 19 kDa.

Recombinant Mouse Keratinocyte Growth Factor is a disulfide-linked monomeric protein consisting of 164 amino acid residue subunits.  Mouse KGF migrates as an approximately 19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>96%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent  proliferation of BAF3 cells was found to be less than 10 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MHKWILTWIL PTLLYRSCFH IICLVGTISL ACNDMTPEQM ATNVNCSSPE RHTRSYDYME 

        70         80         90        100        110        120 
GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNNYNIMEI RTVAVGIVAI KGVESEFYLA 

       130        140        150        160        170        180 
MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHNG GEMFVALNQK GIPVRGKKTK 

       190 
KEQKTAHFLP MAIT 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant KGF was lyophilized from a 0.2 μm filtered 1M NaCl, 20mM PB solution pH 8.0.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

KGF

KGF is a member of the family of fibroblast growth factors, it is secreted in large amounts by fibroblast- like stromal cells in epithelial tissues. The KGF receptor encodes a tyrosine kinase and is a member of the family of receptors binding aFGF and bFGF. Binding of KGF to its receptor is competed by aFGF and bFGF. Although KGF is a potent specific mitogen for many epithelial cells it is not so for fibroblasts and endothelial cells. KGF is thought to play an important role in the paracrine growth control of normal epithelial cells. KGF stimulates the proliferation of primary and secondary human keratinocytes to the same extent as EGF.

Related Publications:
influence of KGF on the progression of pancreatic cancer
Anticancer Res, Aug 2009; 29: 3417 - 3420.
keratinocyte growth factor (KGF) regulates estrogen receptor- (er-) expression and cell apoptosis via phosphatidylinositol 3-kinase (pi3k)/akt pathway in human breast cancer cells
Anticancer Res, Aug 2009; 29: 3195 - 3205.
KGF and egf signalling block hair follicle induction and promote interfollicular epidermal fate in developing mouse skin
Development, Jul 2009; 136: 2153 - 2164.
sirna inhibition of er- expression reduces KGF-induced proliferation of breast cancer cells
Anticancer Res, Sep 2008; 28: 2733 - 2735.
effects of KGF on alveolar epithelial cell transdifferentiation are mediated by jnk signaling
Am. J. Respir. Cell Mol. Biol., Feb 2008; 38: 239 - 246.




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