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Mouse Leukemia Inhibitory Factor Recombinant

LIF

accession:  P09056
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 3500
Domain  :  4 helix cytokine core
Gene  :  LIF
Catalog no. :  RKP09056
Source:
Optimized DNA sequence encoding Mouse LIF mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native Mouse LIF is generated by the proteolytic removal of the signal peptide
and propeptide,  the molecule has a calculated molecular mass of approximately 20 kDa.

LIF is a disulfide-linked monomer protein consisting of 181 amino acid residue subunits, and migrates as an approximately 20 kDa protein under non-reducing
and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC.

 
Biological Activity:
The ED(50) was determined by the dose-dependent differentiation of M1 myeloid leukemic cells was found to be in the range of 0.01 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MKVLAAGIVP LLLLVLHWKH GAGSPLPITP VNATCAIRHP CHGNLMNQIK NQLAQLNGSA 

        70         80         90        100        110        120 
NALFISYYTA QGEPFPNNVE KLCAPNMTDF PSFHGNGTEK TKLVELYRMV AYLSASLTNI 

       130        140        150        160        170        180 
TRDQKVLNPT AVSLQVKLNA TIDVMRGLLS NVLCRLCNKY RVGHVDVPPV PDHSDKEAFQ 

       190        200 
RKKLGCQLLG TYKQVISVVV QAF 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant  Leukemia Inhibitory Factor was lyophilized from a 0.2 μm filtered PBS pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Leukemia Inhibitory Factor

LIF, a multifunctional, secreted glycoprotein that exists in both soluble and matrix-bound forms, displays biologic activities ranging from the differentiation of myeloid leukemic cells into macrophage lineage to effects on bone metabolism, inflammation, neural development, embryogenesis, and the maintenance of implantation (2). It is now clear that LIF is related in both structure and mechanism of action to the interleukin (IL)-6 family of cytokines, which also includes IL-11, ciliary neurotrophic factor, oncostatin M, and cardiotrophin 1 (2). The actions of these cytokines are mediated through specific cell-surface receptors that consist of a unique chain and the shared signal transducing subunit gp130

Related Publications:
arcuate nucleus proopiomelanocortin neurons mediate the acute anorectic actions of Leukemia Inhibitory Factor via gp130
Endocrinology, Feb 2010; 151: 606 - 616.
neuroprotective and axon growth-promoting effects following inflammatory stimulation on mature retinal ganglion cells in mice depend on ciliary neurotrophic factor and Leukemia Inhibitory Factor
J. Neurosci., Nov 2009; 29: 14334 - 14341.
socs1, socs3, and pias1 promote myogenic differentiation by inhibiting the Leukemia Inhibitory Factor-induced jak1/stat1/stat3 pathway
Mol. Cell. Biol., Sep 2009; 29: 5084 - 5093.
interleukin-11 and Leukemia Inhibitory Factor regulate the adhesion of endometrial epithelial cells: implications in fertility regulation
Endocrinology, Jun 2009; 150: 2915 - 2923.
Leukemia Inhibitory Factor controls a retinal rescue mechanism which supports survival of photoreceptor cells in vivo
Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 3880.




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