///Human MIP-4 (CCL18) Recombinant

Human MIP-4 (CCL18) Recombinant

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$70.00$2,700.00

SKU: RKP55774 Tags: , , , , , , , , , , , , ,

Description

Accession
P55774
Source
Optimized DNA sequence encoding Human MIP-4 (CCL18) mature chain was expressed in Escherichia Coli.
Molecular weight
Human CCL-18, generated by the proteolytic removal of the signal peptide and propeptide and has a calculated molecular mass of approximately 8kDa. Recombinant CCL18 is a monomer protein consisting of 68 amino acid residue subunits and migrates as an approximately16 kDa protein under non-reducing conditions andkDa under reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to chemoattract human T cells using a concentration range of.0-40.0 ng/ml.

Protein Sequence
MKGLAAALLV LVCTMALCSC AQVGTNKELC CLVYTSWQIP QKFIVDYSET SPQCPKPGVI LLTKRGRQIC ADPNKKWVQK YISDLKLNA
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant CCL-18 was lyophilized from a.2 μm filteredmM PB,100mM NaCl solution pH.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Molecular function
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