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Human HCC-2(CCL15) Recombinant

HCC-2

accession:  Q16663
   Size A     5ug   $ 70
   Size B      25ug  $160
   Size C      1mg  $ 2700
Domain  :  IL8
Gene  :  CCL15
Catalog no. :  RKQ16663
Source:
Optimized DNA sequence encoding Human HCC-2 (CCL15) mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Human HCC-2, generated by the proteolytic removal of the signal peptide
and propeptide and has a calculated molecular mass of approximately 8 kDa.

Recombinant HCC-2 is a disulfide-linked monomeric protein consisting of 67 amino acid residue subunits and migrates as an approximately 8 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Determined by its ability to chemoattract human monocytes using a concentration range of 2.0-40.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MKVSVAALSC LMLVAVLGSQ AQFINDAETE LMMSKLPLEN PVVLNSFHFA ADCCTSYISQ 

        70         80         90        100        110 
SIPCSLMKSY FETSSECSKP GVIFLTKKGR QVCAKPSGPG VQDCMKKLKP YSI 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant HCC-2 was lyophilized from a 0.2 μm filtered PBS solution pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

HCC-2

CCL15 is a human chemokine which acts predominantly on chemokine receptor CCR1 and to a lesser degree on CCR3. It is a potent chemoattractant for monocytes and lymphocytes in vitro and, after i.p. application, CCL15 induces recruitment neutrophils, monocytes, and lymphocytes into mouse peritoneum. CCL15 is constitutively expressed in the gut and in the liver and was identified to circulate in human plasma. The N terminus of CCL15 was found to be important to exert its full biological activity. HCC-2 shares significant sequence homology with CKb8 and the murine chemokines C10, CCF18/MRP-2, and macrophage inflammatory protein 1g, which all contain six instead of four conserved cysteines.

Related Publications:
hemofiltrate cc chemokines with unique biochemical properties: hcc-1/ccl14a and HCC-2/ccl15
J. Leukoc. Biol., Sep 2001; 70: 357.




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