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Human HCC-1(CCL14) Recombinant

HCC-1

accession:  Q16627
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 2700
Domain  :  IL8
Gene  :  CCL14
Catalog no. :  RKQ16627
Source:
Optimized DNA sequence encoding Human HCC-1(CCL14) mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Human HCC-1, generated by the proteolytic removal of the signal peptide
and propeptide and has a calculated molecular mass of approximately 9 kDa.

Recombinant HCC-1 is a disulfide-linked monomeric protein consisting of 73 amino acid residue subunits and migrates as an approximately 9 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Determined by its ability to chemoattract human monocytes using a concentration range of 2.0-40.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MKISVAAIPF FLLITIALGT KTESSSRGPY HPSECCFTYT TYKIPRQRIM DYYETNSQCS 

        70         80         90 
KPGIVFITKR GHSVCTNPSD KWVQDYIKDM KEN 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant HCC-1 was lyophilized from a 0.2 μm filtered 20mM PB,100mM NaCl solution pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

HCC-1

Hemofiltrate C-C chemokine (HCC)-1 is a recently described monocyte chemoattractant. CCL14 (also known as HCC-1) belongs to the CC chemokine family. Its mature propeptide is a low- affinity agonist of CCR1 that is converted to a high- affinity agonist of CCR1 and CCR5 on proteolytic processing by serine proteases. Determination of the amino acid sequence of HCC-1 revealed four cysteine residues in positions characteristic of the C-C chemokine family, and comparison with the sequences of other chemokines revealed that HCC-1 was most homologous to MIP-1a. However, several functional properties of HCC-1 were atypical of chemokines. Unlike other chemokines, HCC-1 was expressed constitutively in a number of tissues and was present at high concentrations in normal human plasma. In addition, HCC-1 was reported not to be chemotactic for leukocytes.

Related Publications:
hemofiltrate cc chemokines with unique biochemical properties: HCC-1/ccl14a and hcc-2/ccl15
J. Leukoc. Biol., Sep 2001; 70: 357.
identification of c-c chemokine receptor 1 (ccr1) as the monocyte hemofiltrate c-c chemokine (hcc)-1 receptor
J. Exp. Med., Aug 1998; 188: 603 - 608.




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