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Human EMAP-II Recombinant

EMAP-II

accession:  Q12904
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 3500
Domain  :  tRNA BINDING
Gene  :  AIMP1
Catalog no. :  RKQ12904
Source:
Optimized DNA sequence encoding Human EMAP-II mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native human EMAP-II, generated by the proteolytic removal  by caspase-7 in response to apoptosis to produce EMAP-II, the molecule has a calculated molecular mass of approximately 18kDa.

Recombinant EMAP-II is a disulfide-linked monomer protein consisting of 167 amino acid residue subunits, and migrates as an approximately 18kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by apoptotic effect on MCF-7 cells using a concentration of 10-50 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MANNDAVLKR LEQKGAEADQ IIEYLKQQVS LLKEKAILQA TLREEKKLRV ENAKLKKEIE

        70         80         90        100        110        120 
ELKQELIQAE IQNGVKQIPF PSGTPLHANS MVSENVIQST AVTTVSSGTK EQIKGGTGDE

       130        140        150        160        170        180 
KKAKEKIEKK GEKKEKKQQS IAGSADSKPI DVSRLDLRIG CIITARKHPD ADSLYVEEVD 

       190        200        210        220        230        240 
VGEIAPRTVV SGLVNHVPLE QMQNRMVILL CNLKPAKMRG VLSQAMVMCA SSPEKIEILA 

       250        260        270        280        290        300 
PPNGSVPGDR ITFDAFPGEP DKELNPKKKI WEQIQPDLHT NDECVATYKG VPFEVKGKGV 

       310 
CRAQTMSNSG IK 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant EMAP-II was lyophilized from a 0.2 μm filtered 20mM PB,130mM NaCl solution pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

EMAP-II

EMAP-II is activated during apoptosis by cleavage of pro- EMAP through caspases8 and enhances the recruitment of inflammatory cells by its chemotactic properties. Besides its chemotactic effect on inflammatory cells, EMAP-II increases the adhesiveness of coronary artery smooth muscle cells (CASMC) for monocytes. EMAP- II also has anti-angiogenic properties, which are explained by its pro- apoptotic effect on endothelial cells.

Related Publications:
EMAP-II downregulation contributes to the beneficial effects of rapamycin after vascular injury
Cardiovasc Res, Feb 2008; 77: 580 - 589.
p43/EMAP-II expression in colorectal cancer is assicated with hypoxia and enhanced lymphocyte apoptosis
AACR Meeting Abstracts, Apr 2006; 2006: 1118.
endothelial monocyte-activating polypeptide-ii (EMAP-II): a novel inducer of lymphocyte apoptosis
J. Leukoc. Biol., May 2004; 75: 772 - 776.
in vivo antiendothelial EMAP-II therapy in a pancreatic cancer xenotransplant model
AACR Meeting Abstracts, Mar 2004; 2004: 14 - 15.
structure and regulation of the endothelial monocyte activating polypeptide ii (EMAP-II) gene
AACR Meeting Abstracts, Mar 2004; 2004: 159.




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