Human Interleukin-31 Recombinant
Categories: IL-6 gp130 familyRecombinant Human Cytokines$70.00 – $4,700.00
Description
Accession
Q6EBC2
Source
Optimized DNA sequence encoding Human Interleukin-31 mature chain was expressed in Escherichia Coli.
Molecular weight
Native human Interleukin-31, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 16kDa. Recombinant Interleukin-31 is a monomer protein consisting of 141 amino acid residue subunits,and migrates as an approximately 16kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the ability to induce STAT3 activation in U87 MG cells, and was determined to be not less thanng/ml.
Protein Sequence
MASHSGPSTS VLFLFCCLGG WLASHTLPVR LLRPSDDVQK IVEELQSLSK MLLKDVEEEK GVLVSQNYTL PCLSPDAQPP NNIHSPAIRA YLKTIRQLDN KSVIDEIIEH LDKLIFQDAP ETNISVPTDT HECKRFILTI SQQFSECMDL ALKSLTSGAQ QATT
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Interleukin-33 was lyophilized from a 0.2 μm filtered PBS solution pH7.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
