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Mouse Interleukin-33 Recombinant

IL-33

accession:  Q8BVZ5
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 4700
Domain  : 
Gene  :  IL33
Catalog no. :  RKQ8BVZ5
Source:
Optimized DNA sequence encoding Mouse Interleukin-33 mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native Mouse Interleukin-33, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 18kDa.

Recombinant Interleukin-33 is a disulfide-linked monomer protein consisting of 159 amino acid residue subunits, and migrates as an approximately 18kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent  proliferation of murine D10S cells is ≤ 0.3 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MRPRMKYSNS KISPAKFSST AGEALVPPCK IRRSQQKTKE FCHVYCMRLR SGLTIRKETS 

        70         80         90        100        110        120 
YFRKEPTKRY SLKSGTKHEE NFSAYPRDSR KRSLLGSIQA FAASVDTLSI QGTSLLTQSP 

       130        140        150        160        170        180 
ASLSTYNDQS VSFVLENGCY VINVDDSGKD QEQDQVLLRY YESPCPASQS GDGVDGKKLM 

       190        200        210        220        230        240 
VNMSPIKDTD IWLHANDKDY SVELQRGDVS PPEQAFFVLH KKSSDFVSFE CKNLPGTYIG 

       250        260 
VKDNQLALVE EKDESCNNIM FKLSKI 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Interleukin-33 was lyophilized from a 0.2 μm filtered PBS solution pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

IL-33

IL-33, a member of the IL-1 family, signals via a heterodimeric receptor complex consisting of ST2 and IL-1R accessory protein and triggers the activation of NF-B and all three MAPKs: p38, ERK1/2, and JNK1/2 in mast cells.IL-33 is expressed in multiple tissues and by several cell types such as dermal fibroblasts and small airway epithelial and bronchial smooth muscle cells. IL-33 activated IL-1-like signaling responses in mast cells and enhanced IL-5 and IL-13 production from murine Th2-polarized splenocytes.Moreover, it was found that both human and murine mast cells when stimulated in vitro with IL- 33 produced a wide spectrum of cytokines and chemokines . In addition, IL-33 enhanced IL-4-driven Th2 cell responses and acted as a selective chemoattractant for Th2 cell recruitment.

Related Publications:
IL-33 induces il-13–dependent cutaneous fibrosis
J. Immunol., Feb 2010; 184: 1526 - 1535.
serum levels of IL-33 and soluble st2 and their association with disease activity in systemic lupus erythematosus
Rheumatology, Dec 2009; 10.1093/rheumatology/kep402.
caspase-1, caspase-8, and calpain are dispensable for IL-33 release by macrophages
J. Immunol., Dec 2009; 183: 7890 - 7897.
IL-33 amplifies the polarization of alternatively activated macrophages that contribute to airway inflammation
J. Immunol., Nov 2009; 183: 6469 - 6477.
IL-33 shifts macrophage polarization, promoting resistance against pseudomonas aeruginosa keratitis
Invest. Ophthalmol. Vis. Sci., Nov 2009; 10.1167/iovs.09-3983.




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