Optimized DNA sequence encoding Human extracellular domain of human CD89 including a C-terminal 6His tag was expressed in HEK293 cells.
Recombinant CD89 is a monomer protein consisting of 217 amino acid residue subunits, due to glycosylation migrates as an approximately 45-50 kDa protein on SDS-PAGE.
>97%, as determined by SDS-PAGE and HPLC
The activity was tested by immobilized recombinant CD89 (10ug/ml) ability to bind human IgA in functional ELISA.
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant CD89 is supplied as a 0.2 μm filtered PBS solution, pH7.2 .
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant CD89, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
FcRI/CD89 is a type I
expressed at the surface of
myeloid cells and member of
the Ig superfamily. CD89
binds both IgA1 and IgA2
with similar affinity. The
site of interaction between
CD89 and IgA was identified
in the first extracellular
domain of CD89 and the C2/C3
junction of IgA. CD89 is
constitutively expressed as
a 50- to 70-kDa protein on
monocytes/macrophages, or as
a 70- to 100-kDa
glycoprotein on eosinophils
due to increased
glycosylation. CD89 is also
expressed on Kupffer cells
in the liver, where it was
suggested to provide a
second line of defense. At
the cell surface, CD89 is
associated with the FcR -
chain through the charged
arginin residue within its
a recombinant bispecific single-chain fragment variable specific for hla class ii and fcri (CD89) recruits polymorphonuclear neutrophils for efficient lysis of malignant b lymphoid cells
1210 - 1217.
inside-out regulation of fcri (CD89) depends on pp2a
4080 - 4088.
a bispecific antibody composed of a nonneutralizing antibody to the gp41 immunodominant region and an anti-CD89 antibody directs broad human immunodeficiency virus destruction by neutrophils
4671 - 4674.
nonhuman primate iga: genetic heterogeneity and interactions with CD89 J. Immunol.,
4816 - 4824.
intracellular pools of fcr (CD89) in human neutrophils are localized in tertiary granules and secretory vesicles, and two fcr isoforms are found in tertiary granules
J. Leukoc. Biol.,
551 - 558.