Optimized DNA sequence encoding Human extracellular domain of human CD106 including a C-terminal 6His tag was expressed in HEK293 cells.
Recombinant CD106 (VCAM1) is a monomer protein consisting of 689 amino acid residue subunits, due to glycosylation migrates as an approximately 110 kDa protein on SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity of recombinant VCAM1 was tested by immobilized recombinant CD106 (10ug/ml) ability to support adhesion of U937 human histiocytic lymphoma cells (5x104 cells/well ) , 70% will adhere after 60min at 37C.
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant CD106 is supplied as a 0.2 μm filtered PBS solution, pH7.2 .
Recombinant CD106, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
differential engagement of modules 1 and 4 of vascular cell adhesion molecule-1 (CD106) by integrins 41 (cd49d/29) and m2 (cd11b/18) of eosinophils
J. Biol. Chem.,
32175 - 32187.
novel expression of vascular cell adhesion molecule-1 (CD106) by squamous epithelium in experimental acute graft-versus-host disease
Am. J. Pathol.,
763 - 770.
vascular cell adhesion molecule-1 (CD106) is cleaved by neutrophil proteases in the bone marrow following hematopoietic progenitor cell mobilization by granulocyte colony-stimulating factor
1289 - 1297.
rheumatoid arthritis synovial stromal cells inhibit apoptosis and up-regulate bcl-xl expression by b cells in a cd49/cd29-CD106-dependent mechanism
1110 - 1116.
thrombin-activated human endothelial cells support monocyte adhesion in vitro following expression of intercellular adhesion molecule-1 (icam-1; cd54) and vascular cell adhesion molecule-1 (vcam-1; CD106)
1259 - 1267.