Optimized DNA sequence encoding Human extracellular domain of human SLAM (CD150) including a C-terminal 6His tag was expressed in HEK293 cells.
Recombinant SLAM(CD150) is a monomer protein consisting of 228 amino acid residue subunits, due to glycosylation migrates as an approximately 45-50 kDa protein on SDS-PAGE.
>97%, as determined by SDS-PAGE and HPLC
The actiity of recombinant SLAM (CD150) was measured by its ability to bind biotinylated recombinant human SH2D1A in a functional ELISA.
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant CD150 is supplied as a 0.2 μm filtered PBS solution, pH7.2 .
Recombinant SLAM (CD150), as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
The SLAM family of receptors
is a group of type I
transmembrane receptors that
includes SLAM (CD150;
SLAMF1), 2B4 (CD244;
SLAMF4), Ly-9 (CD229;
SLAMF3), natural killer, T-
and B-cell antigen (NTB-A)
or Ly108 (in the mouse)
(SLAMF6), CD84 (SLAMF5), and
CD2-like receptor activating
cytotoxic cells (CRACC;
CD319; and SLAMF7).
SLAM-family receptors are
broadly expressed in immune
cells, and are not found in
nonimmune cells. They
possess an extracellular
segment usually composed of
two immunoglobulin (Ig)-like
domains—one variable (V)-
like domain and one constant
2 (C2)-like domain—in
addition to a transmembrane
region and a cytoplasmic
domain bearing multiple
signaling lymphocytic activation molecule (slam, CD150)-blind measles virus is attenuated and induces strong adaptive immune responses in rhesus monkeys
measles virus infection of alveolar macrophages and dendritic cells precedes spread to lymphatic organs in transgenic mice expressing human signaling lymphocytic activation molecule (slam, CD150)
human and rhesus macaque hematopoietic stem cells are not enriched in the CD150+cd48- slam population.
Blood (ASH Annual Meeting Abstracts),
dynamic interaction of the measles virus hemagglutinin with its receptor signaling lymphocytic activation molecule (slam, CD150)
J. Biol. Chem.,
11763 - 11771.
CD150– side population cells represent a functionally distinct population of long-term hematopoietic stem cells
2444 - 2451.