Cart contains    items

Browse by molecule: [A]  [B]  [C]  [D]  [E]  [F]  [G]  [H]  [I]  [J]  [K]  [L]  [M]  [N]  [O]  [P]  [Q]  [R]  [S]  [T]  [U]  [V]  [W]  [X]  [Y]  [Z]  

Human CD80 Recombinant (B7-1)


accession:  P33681
   Size A     50ug   $ 160
   Size B      200ug  $480
   Size C      1000ug  $ 1950
Domain  :  Ig-like C2
Gene  :  B7-1
Catalog no. :  RKP33681

Optimized DNA sequence  encoding Human extracellular domain of human CD80 including a C-terminal 6His tag was expressed in HEK293 cells.

Molecular weight:

Recombinant CD80 is a monomer protein consisting of 214 amino acid residue subunits,  due to glycosylation migrates as an approximately 45 kDa protein on SDS-PAGE.

>90%, as determined by SDS-PAGE and HPLC



Biological Activity:

The activity was tested by immobilized recombinant CD80 (1ug/ml) ability to bind recombinant human CD28 in functional ELISA.

Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Recombinant CD80  was lyophilized from 0.2um filtered 50mM Tris , 100mM NaCl , pH 8.0 .

Recombinant CD80, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

This product is for research purposes only. It may not be used for therapeutics or diagnostic purposes.



CD80 is a monomeric surface protein found on activated APCs. The extracellular region of CD80 consists of two highly glycosylated Ig- like extracellular domains, which have defined regions for binding its ligands, CD28 or CTLA-4, on T cells.One ligand of CD80, CD28, provides critical costimulatory signals to activated T cells to increase cytokine production, cell proliferation, and cell viability, while the other ligand, CTLA-4, may mediate a growth-inhibitory signal.The same regions of CD80 are involved in binding to both CD28 and CTLA-4, but CTLA-4 binds to CD80 with about a 20-fold higher avidity than CD28 does. This suggests that low levels of CTLA-4 expressed on the cell surface can compete for CD80 binding even when CD28 is expressed at much higher levels.

Related Publications:
tumor cell programmed death ligand 1-mediated t cell suppression is overcome by coexpression of CD80
J. Immunol., Jun 2011; 186: 6822 - 6829.
toll-like receptor 3 ligands induce CD80 expression in human podocytes via an nf-b-dependent pathway
Nephrol. Dial. Transplant., May 2011; 10.1093/ndt/gfr271.
trans-endocytosis of CD80 and cd86: a molecular basis for the cell-extrinsic function of ctla-4
Science, Apr 2011; 332: 600 - 603.
critical role of transcription factor pu.1 in the expression of CD80 and cd86 on dendritic cells
Blood, Feb 2011; 117: 2211 - 2222.
enhancement of suboptimal cd8 cytotoxic t cell effector function in vivo using antigen-specific CD80 defective t cells
J. Immunol., Jan 2011; 186: 291 - 304.

by class:
Growth factors
CD markers
Viral Antigens
by species:
Purified Polyclonal
Related Products:
product_icon_8.jpg Human CD80 Recombinant (B7-1)
product_icon_8.jpg Mouse CD80 Recombinant (B7-1)
Item name Size Quantity Price(USD) Remove
Cart Total $