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Monoclonal Mouse anti Human CD4

CD4

accession:  P01730
   Size A       $
   Size B      500ug  $400
   Size C      1mg  $ 600
Clone  :  RKCD4
Isotype  :  IgG1
Catalog no. :  RMA01730
Applications:
  • Western blot: This monoclonal antibody has been tested in WB analysis of human CD4 . A suitable range of concentrations of this antibody for WB detection is 0.5-2 µg/ml.
 
Source:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human CD4. 
 
Species reactivity:
Human
 
Purification:

The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.

 
Presentation:
Lyophilized from sterile filtered solution at a concentration of 1mg/ml in citrate-phosphate buffer pH7.0, with 0.02%Proclin 300 as a preservative.
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. 

 

CD4

CD4 is a 55 kDa transmembrane glycoprotein belonging to the immunoglobulin superfamily. In humans CD4 is expressed of peripheral T-cells, thymocytes, on macrophages and granulocytes. CD4 acts as a cellular adhesion molecule binding MHC class 2 molecules. It stabilizes the interaction of MHC class 2 restricted T- cells and antigen-presenting cells expressing an antigen in combination with a MHC class 2 molecule. Interactions of CD4 with MHC class 2 molecules are crucial during thymic development and subsequently for the function of single- positive CD4(+), CD8(-) T- lymphocytes. CD4 functions as an effector molecule for intracellular signal transduction. In T- lymphocytes CD4 has been found to be associated with tyrosine kinase lck which phosphorylates a number of other cell surface proteins and is involved probably in transducing the intracellular signal when these proteins are bound to their receptors.

Related Publications:
CD4+cd25+ regulatory t cells resist a novel form of cd28- and fas-dependent p53-induced t cell apoptosis
J. Immunol., Jan 2010; 184: 94 - 104.
CD4+ cd25+ foxp3+ t regulatory cells with limited tcr diversity in control of autoimmunity
J. Immunol., Jan 2010; 184: 56 - 66.
early CD4+ t cell help prevents partial cd8+ t cell exhaustion and promotes maintenance of herpes simplex virus 1 latency
J. Immunol., Jan 2010; 184: 277 - 286.
evaluation of CD4-CD4i antibody architectures yields potent, broadly cross-reactive anti-human immunodeficiency virus reagents
J. Virol., Jan 2010; 84: 261 - 269.
leptin: a critical regulator of CD4+ t-cell polarization in vitro and in vivo
Endocrinology, Jan 2010; 151: 56 - 62.




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Cytokines
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by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
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