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Monoclonal Mouse anti Human CD3

CD3

accession:  P07766
   Size A       $
   Size B      500ug  $400
   Size C      1mg  $ 600
Clone  : 
Isotype  :  IgG1
Catalog no. :  RMA07766
Applications:
  • Western blot: This monoclonal antibody has been tested in WB analysis of human CD3  . A suitable range of concentrations of this antibody for WB detection is 0.5-2 µg/ml.
 
Source:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human CD3. 
 
Species reactivity:
Human
 
Purification:

The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.

 
Presentation:
Lyophilized from sterile filtered solution at a concentration of 1mg/ml in citrate-phosphate buffer pH7.0, with 0.02%Proclin 300 as a preservative.
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

CD3



Related Publications:
anti-CD3 antibody decreases inflammation and improves outcome in a murine model of pneumocystis pneumonia
J. Immunol., Jan 2010; 184: 497 - 502.
transient epstein barr virus reactivation in CD3 monoclonal antibody-treated patients
Blood, Dec 2009; 10.1182/blood-2009-02-204875.
expression of fully assembled tcr–CD3 complex on double positive thymocytes: synergistic role for the prs and er retention motifs in the intra-cytoplasmic tail of CD3
Int. Immunol., Dec 2009; 21: 1317 - 1327.
pharmacokinetics and pharmacodynamics of a chimeric/humanized anti-CD3 monoclonal antibody, otelixizumab (trx4), in subjects with psoriasis and with type 1 diabetes mellitus
J. Clin. Pharmacol., Nov 2009; 10.1177/0091270009349376.
immunotherapy with anti-CD3 x anti-cmv bispecific antibody (cmvbi) armed donor derived activated t cells (atc) – a novel strategy against cytomegalovirus (cmv) post allogeneic stem cell transplantation (sct).
Blood (ASH Annual Meeting Abstracts), Nov 2009; 114: 1157.




by class:
Cytokines
Chemokines
Growth factors
CD markers
Viral Antigens
Other
by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
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