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Monoclonal Mouse anti Human CD2

CD2

accession:  P06729
   Size A       $
   Size B      500ug  $400
   Size C      1mg  $ 600
Clone  :  SBCD2
Isotype  :  IgG1
Catalog no. :  RMA06729
Applications:
  • Western blot: This monoclonal antibody has been tested in WB analysis of human CD2  . A suitable range of concentrations of this antibody for WB detection is 0.5-2 µg/ml.
 
Source:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human CD2. 
 
Species reactivity:
Human
 
Purification:

The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.

 
Presentation:
Lyophilized from sterile filtered solution at a concentration of 1mg/ml in citrate-phosphate buffer pH7.0, with 0.02%Proclin 300 as a preservative.
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

CD2

Cell surface molecule CD2 is a 45- to 58-kDa type I integral protein expressed on virtually all T lineage and NK cells. The extracellular domain of CD2 is composed by two Ig superfamily domains, of which the membrane-distal V- like domain is involved in the binding to the ligand. CD2 has different ligands in rodents (CD48) and humans (CD58), and the mechanisms of ligand binding are substantially different, as assessed by thermodynamic analysis. Nevertheless, engagement of CD2 to CD58 in humans, or to CD48 in rodents, facilitates adhesion between T cells and APC and is proposed to promote the formation of an optimal intercellular membrane spacing suitable for TCR recognition of a peptide Ag bound to MHC . Concomitantly with its function as an adhesion molecule, CD2 has a role in the process of signal transduction. It is known that stimulation of CD2 with combinations of mAb or with the physiological ligand induces T cell proliferation in rats and human. Moreover, ligation of CD2 with CD58 augments the IL-12 responsiveness of activated T cells, and can also reverse T cell anergy induced by B7 blockage (10). In contrast, CD2 interacts with the inhibitory receptor CD5, and through this association is able to amplify modulatory signals at the T cell surface

Related Publications:
cd34, cd56 and CD2 are predictive of npm1 mutation, flt3-itd and their favorable prognosis combination in cytogenetically normal acute myeloid leukemia.
Blood (ASH Annual Meeting Abstracts), Nov 2009; 114: 1576.
population pharmacokinetics of siplizumab (medi-507), a humanized anti-CD2 monoclonal antibody, in cancer patients.
Blood (ASH Annual Meeting Abstracts), Nov 2009; 114: 2670.
ex vivo il-2 expansion of cb-nk cells promotes synergistic lfa-1 and CD2 engagement at the nk cell lytic immune synapse; implications for adoptive cb-nk cell therapy in acute myeloid leukemia.
Blood (ASH Annual Meeting Abstracts), Nov 2009; 114: 3029.
inhibition and activation by CD244 depends on CD2 and phospholipase c-1
J. Biol. Chem., Sep 2009; 284: 24725 - 24734.
lfa-1 and CD2 synergize for the erk1/2 activation in the natural killer (nk) cell immunological synapse
J. Biol. Chem., Aug 2009; 284: 21280 - 21287.




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Antibodies:
Purified Polyclonal
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