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Human CD32a Recombinant ( FcgRIIa)

CD32

accession:  P12318
   Size A     20ug   $ 160
   Size B      100ug  $480
   Size C      500ug  $ 1950
Domain  :  Ig-like C2
Gene  :  CD32A
Catalog no. :  RKP12318

Source:
Optimized DNA sequence  encoding Human extracellular domain of human CD32a including a C-terminal 6His tag was expressed in HEK293 cells.

 
Molecular weight:

Recombinant CD32a is a monomer protein consisting of 192 amino acid residue subunits,  due to glycosylation migrates as an approximately 25 kDa protein on SDS-PAGE.


 
Biological Activity:

1ug of Immobilized CD32a  was tested to bind human IgG2 in a range of  10-200ng/ml.

 


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant CD32a  is supplied as a lyophilized 0.2 μm filtered PBS solution,  pH7.2 .

              
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL..It is recommended to add 0.1% BSA for long term storage of reconstituted product.

 
Storage:

Lyophilized CD32a , as supplied, should be stored at -20°C to -70°C

Upon reconstitution , working aliquots may be stored at 2° - 8° C for one month, or  six months at -20C without detectable loss of activity.It is recommended to add 0.1% BSA for long term storage.
 

Avoid repeated freeze/thaw cycles.


 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

CD32a

FcγRIIA is expressed on cells ofboth myeloid and lymphoid lineages as well as on cells of non- hematopoietic origin.Associated with an ITAM(immunoreceptor tyrosine-based activation motif)-bearing adapter subunit,FcRγ,CD32a(FcγRIIA)d elivers an activating signal upon ligand binding,and results in the initiation of inflammatory responses including cytolysis,phagocytosis,degra nulation and cytokine production.The responses can be modulated by signals from the coexpressed inhibitory receptors such as FcγRIIB,and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

Related Publications:
dynamics of the interaction of human igg subtype immune complexes with cells expressing r and h allelic forms of a low-affinity fc receptor CD32a
J. Immunol., Dec 2009; 183: 8216 - 8224.
association of fcriia (CD32a) with lipid rafts regulates ligand binding activity
J. Immunol., Jun 2009; 182: 8026 - 8036.
transgenic expression of human platelet fcriia (CD32a) in mice does not affect the thrombocytopenia associated with wasp deficiency.
Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 1106.




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by species:
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mouse
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Antibodies:
Purified Polyclonal
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