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Human CD16a Recombinant (FcgR3a)

CD16

accession:  P08637
   Size A     20ug   $ 160
   Size B      100ug  $480
   Size C      500ug  $ 1650
Domain  :  Ig-like C2
Gene  :  CD16A
Catalog no. :  RKP08637

Source:
Optimized DNA sequence  encoding Human extracellular domain of human CD16a including a C-terminal 6His tag was expressed in HEK293 cells.

 
Molecular weight:

Recombinant CD16a is a monomer protein consisting of 203 amino acid residue subunits,  due to glycosylation migrates as an approximately 50 kDa protein on SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Recombinant CD16a activity was measured by the ability to bind human IgG with an affinity constant of 180nM.

 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant CD16a  is supplied as a 0.2 μm filtered PBS solution,  pH7.2 .

              
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL.

 
Storage:
Recombinant CD16a, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

CD16a

Human CD16 is encoded by two genes, FcγRIIIA and FcγRIIIB. FcγRIIIa is a transmembranous isoform of the receptor expressed on the surface of natural killer (NK) cells, macrophages, a subset of monocytes and of T cells in association with dimers of the γ-chain of FcεRI and/or ζ-chain of T-cell receptors. FcγRIIIb is a glycosylphosphatidylinositol -anchored isoform restricted to neutrophils. FcγRIII plays a significant role in the clearance of immune complexes, antibody- dependent cellular cytotoxicity of K/NK cells,phagocytosis and antigen presentation.

Related Publications:
affinity and kinetic analysis of fc receptor iiia (CD16a) binding to igg ligands
J. Biol. Chem., Mar 2007; 282: 6210 - 6221.
phagocytosis stimulates mobilization and shedding of intracellular CD16a in human monocytes and macrophages: inhibition by hiv-1 infection
J. Leukoc. Biol., Feb 2006; 79: 294 - 302.




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Antibodies:
Purified Polyclonal
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