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Monoclonal Mouse anti Human CD20

CD20

accession:  P11836
   Size A       $
   Size B      500ug  $400
   Size C      1mg  $ 600
Clone  :  RKCD20
Isotype  :  IgG1
Catalog no. :  RMA11836

 

 

Applications:
  • Western blot: This monoclonal antibody has been tested in WB analysis of human CD20 . A suitable range of concentrations of this antibody for WB detection is 0.5-2 µg/ml.
 
Source:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with human chronic lymphoid leukemia cells.
 
Species reactivity:
Human
 
Purification:

The IgG fraction of the tissue culture supernatant was purified by Protein A affinity chromatography.

 
Presentation:
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C, or at -20° C for six months without detectable loss of activity. 

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. 

 

CD20

CD20 is a signature B-cell differentiation antigen strongly expressed on the surface of mature normal as well as malignant B cells. It is also expressed, but at lower levels and with larger variance, on more immature B cells and their malignant counterparts found in B-cell precursor (BCP) acute lymphoblastic leukemia. The precise function of CD20 remains unknown, it appears to act as part of an ion channel expressed in the plasma membrane,recently shown that CD20 may operate as a store operated calcium (Ca2+) channel facilitating entry of extracellular Ca2+ following BCR-induced emptying of intracellular stores. Interestingly, despite this presumably important role, CD20- deficient mice are without obvious phenotype, which suggests that either this function is not critical for B cell physiology or that molecular redundancy exists.

Related Publications:
antibody-mediated b-cell depletion before adoptive immunotherapy with t cells expressing CD20-specific chimeric t-cell receptors facilitates eradication of leukemia in immunocompetent mice
Blood, Dec 2009; 114: 5454 - 5463.
anti-CD20 therapy in patients with refractory systemic lupus erythematosus: a longitudinal analysis of 52 hispanic patients
Lupus, Dec 2009; 10.1177/0961203309351541.
ms4a1 (CD20) gene expression is down-regulated by recruiting the histone deacetylase protein complex to the promoter in the CD20-negative b-lymphoma cells after treatment with rituximab.
Blood (ASH Annual Meeting Abstracts), Nov 2009; 114: 1286.
protein tomography analysis shows that CD20 molecules bound by different type i and type ii anti-CD20 antibodies are organized in different protein complexes.
Blood (ASH Annual Meeting Abstracts), Nov 2009; 114: 4782.
a phase i study of pro131921, a novel anti-CD20 monoclonal antibody in patients with relapsed/refractory CD20+ indolent nhl: correlation between clinical responses and auc pharmacokinetics.
Blood (ASH Annual Meeting Abstracts), Nov 2009; 114: 3742.




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Antibodies:
Purified Polyclonal
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