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Monoclonal Mouse anti Human CD15

CD15

accession:  P22083
   Size A       $
   Size B      500ug  $400
   Size C      1mg  $ 600
Clone  :  RKCD15
Isotype  :  IgG1
Catalog no. :  RMA22083
Applications:
  • Western blot: This monoclonal antibody has been tested in WB analysis of human CD15 . A suitable range of concentrations of this antibody for WB detection is 0.5-2 µg/ml.
 
Source:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human CD15. 
 
Species reactivity:
Human
 
Purification:

The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.

 
Presentation:
Lyophilized from sterile filtered solution at a concentration of 1mg/ml in citrate-phosphate buffer pH7.0, with 0.02%Proclin 300 as a preservative.
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

CD15

The cell surface carbohydrate Lewis x (CD15) is a well established and important marker for immunophenotyping leukocytes and for immunoregulatory functions.CD15s is a ligand for selectins CD62E and CD62P on activated endothelial cells and platelets.Sialyl Lewis X is synthesized by sequential addition of 2,3-linked sialic acid and 1,3-linked fucose to its N- acetyllactosamine glycoproteins presenting sialyl Lewis X oligosaccharides are specific for each cell-type, providing unique interactions to each cell- type.

Related Publications:
activated cd11b+ CD15+ granulocytes increase in the blood of patients with uveal melanoma
Invest. Ophthalmol. Vis. Sci., Sep 2009; 50: 4295 - 4303.
multipotent CD15+ cancer stem cells in patched-1–deficient mouse medulloblastoma
Cancer Res., Jun 2009; 69: 4682 - 4690.
activated cd11b+ CD15+ granulocytes increase in blood of uveal melanoma patients
Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 3392.
mma monoclonal antibody is a superior anti-CD15 reagent for the diagnosis of classical hodgkin’s lymphoma
Haematologica, May 2007; 92: 708 - 709.
CD15 (lewis x) expression in human myeloid cell differentiation is regulated by sialidase activity.
Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 1931.




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Antibodies:
Purified Polyclonal
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