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Monoclonal mouse anti SCF

SCF

accession:  P21583
   Size A       $
   Size B      500ug  $400
   Size C      1mg  $ 600
Clone  :  four helix cytokine core
Isotype  :  IgG1
Catalog no. :  RMA21583
Applications:
  • ELISA: This antibody can be used at 5 μg/ml with the appropriate secondary reagents to detect human/rat SCF. The detection limit for recombinant SCF is approximately 0.1 ng/well.
 
Source:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human SCF. 
 
Species reactivity:
Human,Rat
 
Purification:

The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.

 
Presentation:
Lyophilized from sterile filtered solution at a concentration of 1mg/ml inPBS pH 7.4
 
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

SCF

SCF is a stromal cell- derived cytokine synthesized by fibroblasts and other cell types. SCF promotes proliferation and early differentiation of cells at the level of multipotential stem cells. It has been suggested that SCF is essential for optimal production of various hematopoietic lineages, mainly because of its ability to prevent apoptosis when it co- stimulates with other cytokines. The receptor for SCF, designated SCFR(CD117), is the oncogene designated as KIT. The biological activities of SCF are synergised considerably by colony stimulating factors GM-CSF and G-CSF, and also by IL7, Epo and some other growth and differentiation factors. In combination with IL7 SCF stimulates the proliferation of pre-B-cells. SCF is also a potent chemoattractant for cells (see also: Chemotaxis), for example, mast cells, expressing the kit receptor. One response to SCF in these cells is a characteristic rearrangement of the actin filaments of the cytoskeleton.

Related Publications:
pollen-specific skp1-like proteins are components of functional SCF complexes and essential for lily pollen tube elongation
Plant Cell Physiol., Aug 2009; 50: 1558 - 1572.
SCF induces -globin gene expression by regulating downstream transcription factor coup-tfii
Blood, Jul 2009; 114: 187 - 194.
jfk, a kelch domain-containing f-box protein, links the SCF complex to p53 regulation
PNAS, Jun 2009; 106: 10195 - 10200.
the extracellular signal-regulated kinase–mitogen-activated protein kinase pathway phosphorylates and targets cdc25a for SCFβ-trcp-dependent degradation for cell cycle arrest
Mol. Biol. Cell, Apr 2009; 20: 2186 - 2195.
mmp9 and SCF protect from apoptosis in acute kidney injury
J. Am. Soc. Nephrol., Apr 2009; 20: 787 - 797.




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mouse
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Antibodies:
Purified Polyclonal
Related Products:
product_icon_7.jpg Monoclonal mouse anti SCF
 
 
 
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