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Human beta Defensin-3 Recombinant

BD-3

accession:  P81534
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2700
Domain  :  Defensin
Gene  :  DEFB103A
Catalog no. :  RKP81534
Source:
Optimized DNA sequence encoding Human beta Defensin-3 mature chain was expressed in Escherichia Coli.

 
Molecular weight:
Native human BD-3 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 5 kDa.

Recombinant beta Defensin-3 is a disulfide-linked monomer protein consisting of 46 amino acid residue subunits, and migrates as an approximately 5 kDa protein under non-reducing and  reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was Determined by its antimicrobial resistance toE.Coli and was determined to be <3 ug/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MRIHYLLFAL LFLFLVPVPG HGGIINTLQK YYCRVRGGRC AVLSCLPKEE QIGKCSTRGR 


KCCRRKK 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Human beta Defensin-3 was lyophilized from 0.2 μm filtered 100mM NaCl, 20mM PB, pH 7.4.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

beta Defensin-3

Defensins are a large family of peptides of which two groups exist in mammals: alpha defensins and beta defensins, which are distinguishable by the spacing and connectivity of the conserved cysteine residues within the mature peptides.It is thought that defensins function in the eradication of pathogens from the host system by inserting themselves into the bacterial membrane under the influence of membrane potential, forming channels which lead to leakage of cytoplasmic molecules and cell death. Unlike hBD-1, this second peptide, human beta-defensin-2 (hBD-2), is regulated at a transcriptional level in response to contact with microorganisms, and is highly eåective in killing the gram negative bacteria Its expression is also upregulated by the cytokine tumor necrosis factor-alpha.

Related Publications:
microbial targeting of 99mtc-labeled recombinant human β-defensin-3 in an animal model of infection: a feasibility pilot study
J. Nucl. Med., May 2009; 50: 823 - 826.
expression of avian β-defensin 3, an antimicrobial peptide, by sperm in the male reproductive organs and oviduct in chickens: an immunohistochemical study
Poult. Sci., Dec 2008; 87: 2653 - 2659.
mouse beta-defensin-14, an antimicrobial ortholog of human beta-defensin-3
Antimicrob. Agents Chemother., May 2008; 52: 1876 - 1879.
wall teichoic acid deficiency in staphylococcus aureus confers selective resistance to mammalian group iia phospholipase a2 and human β-defensin 3
Infect. Immun., May 2008; 76: 2169 - 2176.
analysis and separation of residues important for the chemoattractant and antimicrobial activities of β-defensin 3
J. Biol. Chem., Mar 2008; 283: 6631 - 6639.




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product_icon_8.jpg Mouse beta Defensin-3 Recombinant
product_icon_8.jpg Human beta Defensin-3 Recombinant
 
 
 
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