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Mouse Interleukin-5 Recombinant

IL-5

accession:  P04401
   Size A     5ug   $ 70
   Size B      25ug  $160
   Size C      1mg  $ 4700
Domain  :  four helix cytokine core
Gene  :  IL5
Catalog no. :  RKP04401

Source:
Optimized DNA sequence encoding Mouse Interleukin-5 mature chain was expressed in Insect Cells

 
Molecular weight:

Native Mouse Interleukin-5, generated by the proteolytic removal of the signal peptide
and propeptide, the molecule has a calculated molecular mass of approximately 13 kDa.

Recombinant mouse IL-5 is a disulfide-linked homodimeric protein consisting of two 113 amino acid residue subunits, and migrates as an approximately 26 kDa protein under non-reducing conditions and as a 13 kDa protein under reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent  proliferation of TF-1 cells was ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 6 x 106 units/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MRRMLLHLSV LTLSCVWATA MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ 

        70         80         90        100        110        120 
LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE 

       130 
FLGVMSTEWA MEG 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Interleukin-5 was lyophilized from a 0.2 μm filtered PBS solution.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

IL-5

Interleukin-5 is a specific hematopoietic growth factor that is responsible for the growth and differentiation of eosinophils. IL-5 promotes the growth of immature hematopoietic progenitor cells BFU-E while it causes differentiation of CFU-E the proliferation of which is inhibited by IL-5. IL-5 strongly stimulates the proliferation, cell activation, and differentiation of eosinophils. B-cells can be made responsive to IL5 by treatment with suboptimal doses of IL1. Interleukin-5 also promotes the generation of cytotoxic T-cells from thymocytes. In thymocytes IL-5 induces the expression of high affinity IL2 receptors. In contrast to human Interleukin-5, mouse IL-5 also acts on B-cells. It induces the proliferation of pre-activated B-cells and their differentiation. High affinity and low affinity receptors for IL5 are expressed in all hematopoietic and lymphoid cells.The low affinity receptor, CD125, has a molecular mass of 50 kDa (p60) . The introduction of p60 into these cells generates a high affinity IL-5 receptor. The second subunit of the IL-5 receptor is identical with the larger subunit of the receptor for the colony stimulating factor GM-CSF (CD131). This subunit is involved also in the generation of a high affinity receptor for IL-3 and is being referred to as common beta.

Related Publications:
systemic overexpression of ifn- and IL-5 exacerbates early phase reaction and conjunctival eosinophilia, respectively, in experimental allergic conjunctivitis
Br J Ophthalmol, Aug 2009; 10.1136/bjo.2009.162594.
regulation of myelopoiesis through syntenin-mediated modulation of IL-5 receptor output
Blood, Aug 2009; 10.1182/blood-2009-03-208850.
il-33 induces antigen-specific IL-5+ t cells and promotes allergic-induced airway inflammation independent of il-4
J. Immunol., Oct 2008; 181: 4780 - 4790.
in vitro study of interleukin-5 (IL-5) in human eosinophilic chronic rhinosinusitis cell culture
In Vivo, Sep 2008; 22: 549 - 556.
separate endocytic pathways regulate IL-5 receptor internalization and signaling
J. Leukoc. Biol., Aug 2008; 84: 499 - 509.




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