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Mouse Interleukin-11 Recombinant

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$70.00$160.00

SKU: RKP47873 Tags: , , ,

Description

Accession
P47873
Source
Optimized DNA sequence encoding Mouse Interleukin-11 mature chain was expressed in Escherichia Coli.
Molecular weight
Native Mouse IL-11, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 19 kDa. Recombinant IL-11 is a monomer protein consisting of 180 amino acid residue subunits, and migrates as an approximately 19 kDa protein under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the dose-dependent stimulation of the proliferation ofmurine T11 cells is ≤.0 ng/ml, corresponding to a specific activity of ≥1 xunits/mg.
Protein Sequence
MNCVCRLVLV VLSLWPDRVV APGPPAGSPR VSSDPRADLD SAVLLTRSLL ADTRQLAAQM RDKFPADGDH SLDSLPTLAM SAGTLGSLQL PGVLTRLRVD LMSYLRHVQW LRRAGGPSLK TLEPELGALQ ARLERLLRRL QLLMSRLALP QAAPDQPVIP LGPPASAWGS IRAAHAILGG LHLTLDWAVR GLLLLKTRL
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant mouse IL-11 was lyophilized from a.2 μm filtered PBS solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Molecular function
Molecular function

Methods

Effect of IL-11 on invasion of JEG-3 and HTR-8/SVneo cells.

  • Data is expressed as fold change in invasion following IL-11 (200 ng/ml) treatment as compared to untreated JEG-3 cells as control.

Lentiviral transduction of bone marrow cells

  • Freshly isolated bone marrow (BM) cells were plated in Iscove's Modified Dulbecco's Medium (IMDM) (31980, , ) containing 5% fetal bovine serum (FBS), 1% penicillin/streptomycin, 200 mM glutamine, 1% non-essential amino acids, 1% sodium pyruvate, 50 μM 2-mercaptoethanol, stem cell factor (SCF, 10 ng/ml, , ), Flt3-L (10 ng/ml), IL-11 (10 ng/ml), thrombopoietin (TPO, 10 ng/ml), IL-6 (10 ng/ml), and IL-3 (10 ng/ml).
  • Bone marrow cells were spin-infected with 8 μg/ml polyprene and lentivirus (MOI of 0.1–0.5), at 2,500 rpm for 90 min.
  • BM cells were then incubated for 3 hours at 37°C, counted and injected into NOD.SCID mice.
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