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Mouse Macrophage Colony stimulating Factor Recombinant

M-CSF

accession:  P07141
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 4700
Domain  :  four helical cytokine core
Gene  :  CSF1
Catalog no. :  RKP07141

 

Source:
Optimized DNA sequence encoding Human M-CSF extracellular domain was expressed in Escherichia Coli.

 
Molecular weight:

Mouse MCSF, generated by the proteolytic removal of the signal peptide
and propeptide, The molecule has a calculated molecular mass of approximately 37 kDa.

Recombinant Mouse M-CSF is a disulfide-linked homodimeric protein consisting of two 156 amino acid residue subunits, and migrates as an approximately 37 kDa protein under non-reducing and as 18-19kDa under reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent stimulation of the proliferation of  of M-NFS-60 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MTARGAAGRC PSSTWLGSRL LLVCLLMSRS IAKEVSEHCS HMIGNGHLKV LQQLIDSQME 

        70         80         90        100        110        120 
TSCQIAFEFV DQEQLDDPVC YLKKAFFLVQ DIIDETMRFK DNTPNANATE RLQELSNNLN 

       130        140        150        160        170        180 
SCFTKDYEEQ NKACVRTFHE TPLQLLEKIK NFFNETKNLL EKDWNIFTKN CNNSFAKCSS 

       190    
RDVVTKP
(*)Complete precursor sequence shown, expressed chain highlighted 
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant mouse MCSF was lyophilized from a 0.2 μm filtered PBS solution.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

      

 

M-CSF

M-CSF is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. After cell activation, B-cells and T- cells and also a number of tumor cell lines are capable also of synthesizing this factor. M-CSF has been found to be synthesized by uterine epithelial cells in vivo. The M-CSF receptor is identical with the proto- oncogene fms. It has been renamed CD115. The receptor is a transmembrane protein with an extracellular ligand- binding domain of 512 amino acids, an intramembrane domain of 25 amino acids, and a cytoplasmic domain of 435 amino acids encoding a tyrosine kinase .Human M-CSF is active in mouse and rat cells. The murine factor is active in rat cells but inactive in human cells.M- CSF is a specific factor in that the proliferation inducing activity is more or less restricted to the macrophage lineage. M-CSF is a potent stimulator of functional activities of monocytes.

Related Publications:
gM-CSF and il-4 induce dendritic cell differentiation and disrupt osteoclastogenesis through M-CSF receptor shedding by up-regulation of tnf- converting enzyme (tace)
Blood, Sep 2009; 10.1182/blood-2009-04-215020.
gM-CSF- and M-CSF-dependent macrophage phenotypes display differential dependence on type i interferon signaling
J. Leukoc. Biol., Aug 2009; 86: 411 - 421.
M-CSF inhibition selectively targets pathological angiogenesis and lymphangiogenesis
J. Exp. Med., May 2009; 206: 1089 - 1102.
c-reactive protein induces M-CSF release and macrophage proliferation
J. Leukoc. Biol., Feb 2009; 85: 262 - 267.
M-CSF preferentially induces c-fos via erk to specify monopoiesis whereas g-csf directs granulopoiesis via shp2
Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 2887.




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