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Mouse beta Nerve Growth Factor Recombinant

NGF

accession:  P01139
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2700
Domain  :  NGF
Gene  :  NGF
Catalog no. :  RKP01139

Source:
Optimized DNA sequence encoding Mouse Nerve Growth Factor mature chain was expressed in CHO.

 
Molecular weight:

Native Mouse Nerve Growth Factor is generated by the proteolytic removal of the signal peptide and propeptide the molecule has a calculated molecular mass of approximately 13 kDa.

Recombinant Mouse Nerve Growth Factor  is a  monomeric protein consisting of  120 amino acid residue subunits, and migrates as an approximately 13 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the determined by its ability to stimulate chick E9 DRG neurite outgrowth was found to be in the range of 1.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MSMLFYTLIT AFLIGVQAEP YTDSNVPEGD SVPEAHWTKL QHSLDTALRR ARSAPTAPIA 

        70         80         90        100        110        120 
ARVTGQTRNI TVDPRLFKKR RLHSPRVLFS TQPPPTSSDT LDLDFQAHGT IPFNRTHRSK 

       130        140        150        160        170        180 
RSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR 

       190        200        210        220        230        240 
ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR 


G 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant mouse NGF was lyophilized from a 0.2 μm filtered solution in 10mM sodium acetate, pH 5.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

NGF

NGF is mainly responsible for the survival and the differentiation and the functional activities of sensory and sympathetic neurons in the peripheral nervous system. It also plays an important role in the development and functional activities of cholinergic neurons in the central nervous system. Since NGF is synthesized also in non-neuronal tissues it may have a much wider spectrum of biological activities than thought previously. NGF stimulates chemotactic migration of human polymorphonuclear leukocytes in vitro. NGF stimulates the growth and differentiation of B- cells and the growth of T- cells and of some tumor cell types. NGF inhibits immunoglobulin production by various human plasma cell. The cytokines IL1, IL6, and bFGF are potent inducers of NGF. NGF induces the synthesis of IL1 in pheochromocytoma cells which in turn acts as a growth factor for glial cells and induces the synthesis of NGF following nerve injuries. In thymic stromal cells NGF induces the synthesis of IL6. NGF induces the synthesis of the fos oncogene and the myc oncogene and also influences the expression of EGF. One receptor that is responsible for mediating most of the activities of NGF is expressed preferentially in neuronal tissues. This glycoprotein of 140 kDa is the product of the trk gene. It possesses an intrinsic tyrosine- specific protein kinase in its intracellular domain.

Related Publications:
NGF is essential for hippocampal plasticity and learning
J. Neurosci., Sep 2009; 29: 10883 - 10889.
the cd38/cadpr/ca2+-pathway promotes cell proliferation and delays NGF-induced differentiation in pc12 cells
J. Biol. Chem., Aug 2009; 10.1074/jbc.M109.049767.
nucleocytoplasmic shuttling of the adapter protein sh2b1β (sh2-bβ) is required for nerve growth factor (NGF)-dependent neurite outgrowth and enhancement of expression of a subset of NGF-responsive genes
Mol. Endocrinol., Jul 2009; 23: 1077 - 1091.
prolyl hydroxylase inhibitors depend on extracellular glucose and hypoxia-inducible factor (hif)-2 to inhibit cell death caused by nerve growth factor (NGF) deprivation: evidence that hif-2 has a role in NGF-promoted survival of sympathetic neurons
Mol. Pharmacol., May 2009; 75: 1198 - 1209.
paraplegia increased cardiac NGF content, sympathetic tonus, and the susceptibility to ischemia-induced ventricular tachycardia in conscious rats
Am J Physiol Heart Circ Physiol, May 2009; 296: H1364 - H1372.




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