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Human Bone Morphogenetic Protein-3b Recombinant

BMP-3

accession:  P55107
   Size A     5ug   $ 70
   Size B      25ug  $160
   Size C      1mg  $ 3500
Domain  :  TGFB
Gene  :  BMP3B
Catalog no. :  RKP12645

 

Source:
Optimized DNA sequence encoding Human Bone Morphogenetic Protein-3b mature chain was expressed in Escherichia Coli.
 
Molecular weight:

Native human Bone Morphogenetic Protein-3b is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately  13 kDa.

Recombinant BMP-3b is a disulfide-linked monomer protein consisting of  111 amino acid residue subunits. BMP3 migrates as an approximately 12-13 kDa protein under reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Protein Sequence:
        10         20         30         40         50         60 
MAHVPARTSP GPGPQLLLLL LPLFLLLLRD VAGSHRAPAW SALPAAADGL QGDRDLQRHP 

        70         80         90        100        110        120 
GDAAATLGPS AQDMVAVHMH RLYEKYSRQG ARPGGGNTVR SFRARLEVVD QKAVYFFNLT 

       130        140        150        160        170        180 
SMQDSEMILT ATFHFYSEPP RWPRALEVLC KPRAKNASGR PLPLGPPTRQ HLLFRSLSQN 

       190        200        210        220        230        240 
TATQGLLRGA MALAPPPRGL WQAKDISPIV KAARRDGELL LSAQLDSEER DPGVPRPSPY 

       250        260        270        280        290        300 
APYILVYAND LAISEPNSVA VTLQRYDPFP AGDPEPRAAP NNSADPRVRR AAQATGPLQD 

       310        320        330        340        350        360 
NELPGLDERP PRAHAQHFHK HQLWPSPFRA LKPRPGRKDR RKKGQEVFMA ASQVLDFDEK 

       370        380        390        400        410        420 
TMQKARRKQW DEPRVCSRRY LKVDFADIGW NEWIISPKSF DAYYCAGACE FPMPKIVRPS 

       430        440        450        460        470        480 
NHATIQSIVR AVGIIPGIPE PCCVPDKMNS LGVLFLDENR NVVLKVYPNM SVDTCACR 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant BMP-3b  was lyophilized from a 0.2 μm filtered 1mg/ml 10 mM sodium citrate (pH 3.5) containing 10% glycerol.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
 

BMP3

BMPs are proteins that act to induce the differentiation of mesenchymal-type cells into chondrocytes and osteoblasts before initiating bone formation. They promote the differentiation of cartilage-forming cells and bone-forming cells near sites of fractures but also at ectopic locations. Some of the proteins induce the synthesis of alkaline phosphatase and collagen in osteoblasts. Some BMPs act directly on osteoblasts and promote their maturation while at the same time suppressing myogenous differentiation. Other BMPs promote the conversion of typical fibroblasts into chondrocytes and are capable also of inducing the expression of an osteoblast phenotype in non-osteogenic cell types. Intracellular signaling following engagement of receptors for some BMP proteins has been shown to involve the action of SMAD proteins. BMP3 is a glycoprotein that is identical with Osteogenin. Human and rat mature proteins are 98 % identical.

 




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