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Human CD40 Ligand Recombinant

CD40 Ligand

accession:  P29965
   Size A     10ug   $ 70
   Size B      50ug  $160
   Size C      1mg  $ 1350
Domain  :  TNF
Gene  :  CD40LG
Catalog no. :  RKP29965

Source:
Optimized DNA sequence encoding Human CD40L TNF domain was expressed in Escherichia Coli.

 
Molecular weight:

Recombinant CD40 Ligand is a disulfide-linked monomer protein consisting of 150 amino acid residue subunits, and migrates as an approximately 16 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent stimulation of IL-8 production by human PBMC and was found to be in the range of 5.0-20.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MIETYNQTSP RSAATGLPIS MKIFMYLLTV FLITQMIGSA LFAVYLHRRL DKIEDERNLH

        70         80         90        100        110        120 
EDFVFMKTIQ RCNTGERSLS LLNCEEIKSQ FEGFVKDIML NKEETKKENS FEMQKGDQNP

       130        140        150        160        170        180 
QIAAHVISEA SSKTTSVLQW AEKGYYTMSN NLVTLENGKQ LTVKRQGLYY IYAQVTFCSN

       190        200        210        220        230        240
REASSQAPFI ASLCLKSPGR FERILLRAAN THSSAKPCGQ QSIHLGGVFE LQPGASVFVN

       250        260
VTDPSQVSHG TGFTSFGLLK L

 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
CD40 ligand was lyophilized from a 0.2 μm filtered PBS solution pH 7.0.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

CD40 Ligand

CD40 protein is expressed on all B-lymphocytes during various stages of development, activated T- cells and monocytes, follicular dendritic cells, thymic epithelial cells, and various carcinoma cell lines. It is expressed on most mature B-cell malignancies and on some early B-cell acute lymphocytic leukemias. Human CD40 has been found to be the receptor for a protein involved in an X- linked immunodeficiency syndrome. The CD40 ligand is identical with a T-cell restricted cell surface activation antigen designated 5c8. The mouse ligand of CD40 is gp39.Based on its homology with other members of the TNF ligand protein superfamily the protein is referred to also as TNFSF5.

Related Publications:
the cd40/CD40 Ligand system: linking inflammation with atherothrombosis
J. Am. Coll. Cardiol., Aug 2009; 54: 669 - 677.
growth inhibition of human multiple myeloma cells by an oncolytic adenovirus carrying the CD40 Ligand transgene
Clin. Cancer Res., Aug 2009; 15: 4847 - 4856.
matrix metalloproteinase 9 is involved in crohn’s disease-associated platelet hyperactivation through the release of soluble CD40 Ligand
Gut, Jul 2009; 58: 920 - 928.
distinct roles of cd28- and CD40 Ligand-mediated costimulation in the development of protective immunity and pathology during chlamydia muridarum urogenital infection in mice
Infect. Immun., Jul 2009; 77: 3080 - 3089.
phase i study of intranodal direct injection of adenovirus encoding recombinant cd40-ligand (ad-isf35) in patients with chronic lymphocytic leukemia
ASCO Meeting Abstracts, May 2009; 27: 3003.




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