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Mouse Interleukin-1 beta Recombinant

IL-1

accession:  P10749
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 4700
Domain  :  IL1
Gene  :  IL1B
Catalog no. :  RKP10749

Source:
Optimized DNA sequence encoding Mouse Interleukin-1 beta mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Mature Mouse IL-1 beta, is generated by the proteolytic removal of the signal peptide
and propeptide.The molecule has a calculated molecular mass of approximately 17 kDa.

Recombinant IL-1 beta is a disulfide-linked monomer protein consisting of two 153 amino acid residue subunits.  Recombinant IL-1 beta migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent stimulation of thymidine uptake by murine D10S cells is ≤ 2.0 pg/ml, corresponding to a specific activity of ≥5 x 108 units/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MATVPELNCE MPPFDSDEND LFFEVDGPQK MKGCFQTFDL GCPDESIQLQ ISQQHINKSF 

        70         80         90        100        110        120 
RQAVSLIVAV EKLWQLPVSF PWTFQDEDMS TFFSFIFEEE PILCDSWDDD DNLLVCDVPI 

       130        140        150        160        170        180 
RQLHYRLRDE QQKSLVLSDP YELKALHLNG QNINQQVIFS MSFVQGEPSN DKIPVALGLK 

       190        200        210        220        230        240 
GKNLYLSCVM KDGTPTLQLE SVDPKQYPKK KMEKRFVFNK IEVKSKVEFE SAEFPNWYIS 

       250        260        270 
TSQAEHKPVF LGNNSGQDII DFTMESVSS 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Mouse IL-1 beta was lyophilized from a 0.2 μm filtered solution in PBS, pH 7.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

IL-1 beta

Monocytes are the main source of secreted IL1. They express predominantly IL1- beta while human keratinocytes express large amounts of IL1-alpha. Murine macrophages display a transition from IL1-beta to IL1-alpha production during maturation of monocytes into inflammatory macrophages.There are two functionally almost equivalent forms of IL1, IL1-alpha and IL1-beta that are encoded by two different genes. IL1-beta is the predominant form in humans while it is IL1-alpha in mice. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL1-beta but not the IL1-alpha precursor must be processed before it can bind to the receptor. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T-cells is expressed predominantly on T-cells and cells of mesenchymal origin. It binds both types of IL1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B-cells, granulocytes, and macrophages. It is expressed predominantly on B-cells and cells of the myelomonocytic lineage and is encoded by a separate gene.

Related Publications:
il-1β in aqueous humor induces fgf-2 synthesis in rabbit corneal endothelium through pi 3-kinase and p38
Invest. Ophthalmol. Vis. Sci., Sep 2009; 10.1167/iovs.09-4240.
a high resolution nmr-based model for the structure of a scfv-il-1β complex: potential for nmr as a key tool in therapeutic antibody design and development
J. Biol. Chem., Sep 2009; 10.1074/jbc.M109.025304.
pro-interleukin (il)-1β shares a core region of stability as compared with mature il-1β while maintaining a distinctly different configurational landscape: a comparative hydrogen/deuterium exchange mass spectrometry study
J. Biol. Chem., Sep 2009; 284: 26137 - 26148.
cholesterol-dependent cytolysins induce rapid release of mature il-1 from murine macrophages in a nlrp3 inflammasome and cathepsin b-dependent manner
J. Leukoc. Biol., Aug 2009; 10.1189/jlb.0309164.
pyrin critical to macrophage il-1β response to francisella challenge
J. Immunol., Jun 2009; 182: 7982 - 7989.




by class:
Cytokines
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by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
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