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Rat Tumor Necrosis Factor-alpha Recombinant

TNF

accession:  P16599
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2700
Domain  :  tnf
Gene  :  TNF
Catalog no. :  RKP16599
Source:
Optimized DNA sequence encoding Rat Tumor Necrosis Factor-alpha mature chain was expressed in Escherichia Coli.

 
Molecular weight:
Native Rat TNF-alpha, generated by the proteolytic removal of the signal peptide
and propeptide,the molecule has a calculated molecular mass of approximately 17 kDa.

Recombinant  Rat Tumor Necrosis Factor-alpha is a disulfide-linked monomeric protein consisting of 157 amino acid residue subunits, and migrates as an approximately 17 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20.0-40.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MSTESMIRDV ELAEEALPKK MGGLQNSRRC LCLSLFSFLL VAGATTLFCL LNFGVIGPNK

        70         80         90        100        110        120 
EEKFPNGLPL ISSMAQTLTL RSSSQNSSDK PVAHVVANHQ AEEQLEWLSQ RANALLANGM 

       130        140        150        160        170        180 
DLKDNQLVVP ADGLYLIYSQ VLFKGQGCPD YVLLTHTVSR FAISYQEKVS LLSAIKSPCP 

       190        200        210        220        230 
KDTPEGAELK PWYEPMYLGG VFQLEKGDLL SAEVNLPKYL DITESGQVYF GVIAL 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant rat TNF-a  was lyophilized from a 0.2 μm filtered PBS solution pH 7.0.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

TNF-alpha

TNF is secreted by macrophages, monocytes, neutrophils, T-cells, NK- cells following their stimulation by bacterial lipopolysaccharides. Cells expressing CD4 secrete TNF- alpha while CD8(+) cells secrete little or no TNF- alpha. Stimulated peripheral neutrophilic granulocytes but also unstimulated cells and also a number of transformed cell lines, astrocytes, microglial cells, smooth muscle cells, and fibroblasts also secrete TNF. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, GM-CSF, SP, Bradykinin, Immune complexes, inhibitors of cyclooxygenase and PAF (platelet activating factor). Human TNF-alpha is a non- glycosylated protein of 17 kDa and a length of 157 amino acids. Murine TNF- alpha is N-glycosylated. Homology with TNF-beta is approximately 30 %. TNF- alpha forms dimers and trimers.

Related Publications:
tak1-mediated serine/threonine phosphorylation of epidermal growth factor receptor via p38/extracellular signal-regulated kinase: nf-b-independent survival pathways in tumor necrosis factor alpha signaling
Mol. Cell. Biol., Oct 2009; 29: 5529 - 5539.
tumor necrosis factor--mediated regulation of the inositol 1,4,5-trisphosphate receptor promoter
J. Biol. Chem., Oct 2009; 284: 27557 - 27566.
tnf- inhibition attenuates adverse myocardial remodeling in a rat model of volume overload
Am J Physiol Heart Circ Physiol, Oct 2009; 297: H1462 - H1468.
tnf inhibits apoptotic cell clearance in the lung, exacerbating acute inflammation
Am J Physiol Lung Cell Mol Physiol, Oct 2009; 297: L586 - L595.
association of tnf- gene polymorphisms with systemic lupus erythematosus in taiwanese patients
Lupus, Oct 2009; 18: 974 - 979.




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