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Rat Interleukin 10 Recombinant

IL-10

accession:  P29456
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 4700
Domain  :  four helix cytokine core
Gene  :  IL10
Catalog no. :  RKP29456
Source:
Optimized DNA sequence encoding rat  Interleukin-10 mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native Rat  Interleukin-10 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 19 kDa.

Recombinant Rat Interleukin-10 is a disulfide-linked monomer protein consisting of 161 amino acid residue subunits, and migrates as an approximately 19 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent inhibition of antigen-specific T cell proliferation is less than 12 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MPGSALLCCL LLLAGVKTSK GHSIRGDNNC THFPVSQTHM LRELRAAFSQ VKTFFQKKDQ 

        70         80         90        100        110        120 
LDNILLTDSL LQDFKGYLGC QALSEMIKFY LVEVMPQAEN HGPEIKEHLN SLGEKLKTLW 

       130        140        150        160        170        180 
IQLRRCHRFL PCENKSKAVE QVKNDFNKLQ DKGVYKAMNE FDIFINCIEA YVTLKMKN 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant rat Interleukin-10 was lyophilized from a 0.2 μm filtered PBS solution.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

IL-10

IL-10 is produced by mouse Th2-cells following their stimulation by lectins. The main source for B-cell derived IL10 in mice are Ly1 B-cells that express CD5 (Ly1) and CD11.In humans IL10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones after both antigen-specific and polyclonal activation, by B- cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells.IL10 is a homodimeric protein with subunits having a length of 160 amino acids. Human Interleukin10 shows 73 % amino acid homology with mouse Interleukin-10. The murine IL-10 receptor has been cloned. This receptor is a protein of approximately 110 kDa that binds murine IL10 specifically. This receptor is structurally related to receptors for IFN. The CD nomenclature for this receptor is CDw210. IL10 inhibits the synthesis of a number of cytokines such as IFN-gamma, IL2 and TNF-beta in Th1 T-helper subpopulations of T-cells but not of Th2 T-helper cells. This activity is antagonized by IL4. The inhibitory effect on IFN- gamma production is indirect and appears to be the result of a suppression of IL12 synthesis by accessory cells. In the human system, IL10 is produced by, and down-regulates the function of, Th1 and Th2 cells.

Related Publications:
anti-ctla-4 treatment induces IL-10-producing icos+ regulatory t cells displaying ido-dependent anti-inflammatory properties in a mouse model of colitis
Gut, Oct 2009; 58: 1363 - 1373.
c-jun controls the ability of il-12 to induce IL-10 production from human memory cd4+ t cells
J. Immunol., Oct 2009; 183: 4475 - 4482.
il-27 regulates IL-10 and il-17 from cd4+ cells in nonhealing leishmania major infection
J. Immunol., Oct 2009; 183: 4619 - 4627.
activating receptors promote nk cell expansion for maintenance, IL-10 production, and cd8 t cell regulation during viral infection
J. Exp. Med., Sep 2009; 206: 2235 - 2251.
inhibition of arterial lesion progression in cd16-deficient mice: evidence for altered immunity and the role of IL-10
Cardiovasc Res, Sep 2009; 10.1093/cvr/cvp300.




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