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Rat Interleukin-6 Recombinant

IL-6

accession:  P20607
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 4700
Domain  :  four helical cytokine core
Gene  :  IL6
Catalog no. :  RKP20607
Source:
Optimized DNA sequence encoding rat Interleukin-6 mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native rat Interleukin-6 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 22 kDa.

Recombinant rat Interleukin-6 is a disulfide-linked monomer protein consisting of 188 amino acid residue subunits, and migrates as an approximately 22 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20.0-40.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MKFLSARDFQ PVAFLGLMLL TATAFPTSQV RRGDFTEDTT HNRPVYTTSQ VGGLITYVLR 

        70         80         90        100        110        120 
EILEMRKELC NGNSDCMNSD DALSENNLKL PEIQRNDGCF QTGYNQEICL LKICSGLLEF 

       130        140        150        160        170        180 
RFYLEFVKNN LQDNKKDKAR VIQSNTETLV HIFKQEIKDS YKIVLPTPTS NALLMEKLES 

       190        200        210 
QKEWLRTKTI QLILKALEEF LKVTMRSTRQ T 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant rat Interleukin-6 was lyophilized from a 0.2 μm filtered PBS solution.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

IL-6

IL6 is produced by many different cell types. The main sources in vivo are stimulated monocytes, fibroblasts, and endothelial cells. Macrophages, T-cells and B-lymphocytes, granulocytes, smooth muscle cells, eosinophils, chondrocytes, osteoblasts, mast cells, glial cells, and keratinocytes also produce IL6 after stimulation. IL6 is a member of a family of cytokines, which also includes LIF, CNTF, Oncostatin M, IL11, and CT- 1. All known members of the IL6 cytokine family induce hepatic expression of acute phase proteins. The IL6 receptor is expressed on T-cells, mitogen-activated B-cells, peripheral monocytes and some macrophage- and B-cell derived tumor cell types. It is not expressed in resting B-cells but in resting T- cells. The IL6 receptor is a strongly glycosylated protein of 80 kDa and a length of 449 amino acids. It has been designated CD126.

Related Publications:
IL-6 protects against hyperoxia-induced mitochondrial damage via bcl-2–induced bak interactions with mitofusions
Am. J. Respir. Cell Mol. Biol., Oct 2009; 41: 385 - 396.
long-term safety and efficacy of tocilizumab, an anti-IL-6 receptor monoclonal antibody, in monotherapy, in patients with rheumatoid arthritis (the stream study): evidence of safety and efficacy in a 5-year extension study
Ann Rheum Dis, Oct 2009; 68: 1580 - 1584.
the second bovine β-galactoside-2,6-sialyltransferase (st6gal ii): genomic organization and stimulation of its in vitro expression by IL-6 in bovine mammary epithelial cells
Glycobiology, Oct 2009; 19: 1082 - 1093.
IL-6 -174g/c promoter polymorphism in acute appendicitis
Lab Med, Oct 2009; 40: 600 - 603.
cell surface-bound il-1 is an upstream regulator of the senescence-associated IL-6/il-8 cytokine network
PNAS, Sep 2009; 10.1073/pnas.0905299106.




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