Cart contains    items
SEARCH

  |
  |  
  |  
  |  
  |  
  |  
 
Browse by molecule: [A]  [B]  [C]  [D]  [E]  [F]  [G]  [H]  [I]  [J]  [K]  [L]  [M]  [N]  [O]  [P]  [Q]  [R]  [S]  [T]  [U]  [V]  [W]  [X]  [Y]  [Z]  

Mouse Interleukin-3 Recombinant

IL-3

accession:  P01586
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 4700
Domain  :  four helix cytokine core
Gene  :  IL3
Catalog no. :  RKP01586
Source:
Optimized DNA sequence encoding mouse Interleukin-3 mature chain was expressed in Escherichia Coli.

 
Molecular weight:
Native mouse Interleukin-3 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 15 kDa.

Recombinant mouse IL-3 is a disulfide-linked monomer consisting of 135 amino acid residue subunits, and migrates as an approximately 15 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent stimulation of the proliferation of mouse M-NFS-60 cells was found to be in the <0.05 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MVLASSTTSI HTMLLLLLML FHLGLQASIS GRDTHRLTRT LNCSSIVKEI IGKLPEPELK 

        70         80         90        100        110        120 
TDDEGPSLRN KSFRRVNLSK FVESQGEVDP EDRYVIKSNL QKLNCCLPTS ANDSALPGVF 

       130        140        150        160 
IRDLDDFRKK LRFYMVHLND LETVLTSRPP QPASGSVSPN RGTVEC 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant mouse Interleukin-3 was lyophilized from a 0.2 μm filtered PBS solution.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

IL-3

IL3 is produced mainly by T- cells following cell activation by antigens and mitogens, but also by keratinocytes, NK-cells, mast cells, endothelial cells, and monocytes. The analysis of bacterial- derived recombinant IL3 shows that glycosylation is not required for the activity of IL3. IL3 sequences are evolutionarily less well conserved. Human and murine IL3 show approximately 29 % homology at the protein level while murine and rat IL3 show approximately 54 % homology. IL3-alpha and IL3- beta are two isoforms of rat IL3. IL3 receptors are expressed on macrophages, mast cells, eosinophils, megakaryocytes, basophils, bone marrow progenitor cells, and various myeloid leukemia cells. IL3/receptor complexes have a Kdis of 10-9 - 10-10 M. Binding of IL3 to its receptor causes specific phosphorylation of a 150 kDa membrane glycoprotein.

Related Publications:
ship represses the generation of IL-3-induced m2 macrophages by inhibiting il-4 production from basophils
J. Immunol., Sep 2009; 183: 3652 - 3660.
IL-3 induces basophil expansion in vivo by directing granulocyte-monocyte progenitors to differentiate into basophil lineage-restricted progenitors in the bone marrow and by increasing the number of basophil/mast cell progenitors in the spleen
J. Immunol., Mar 2009; 182: 2835 - 2841.
human basophils secrete IL-3: evidence of autocrine priming for phenotypic and functional responses in allergic disease
J. Immunol., Feb 2009; 182: 2432 - 2438.
14-3-3 is stimulated by IL-3 and promotes cell proliferation
J. Immunol., Jan 2009; 182: 1050 - 1060.
IL-3 inhibits tnf--induced bone resorption and prevents inflammatory arthritis
J. Immunol., Jan 2009; 182: 361 - 370.




by class:
Cytokines
Chemokines
Growth factors
CD markers
Viral Antigens
Other
by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
Related Products:
product_icon_8.jpg Human Interleukin-3 Recombinant
product_icon_8.jpg Human Interleukin-31 Recombinant
product_icon_8.jpg Mouse Interleukin-3 Recombinant
product_icon_7.jpg Rabbit anti Human IL32 alpha Polyclonal
product_icon_8.jpg Human Interleukin-33 Recombinant
product_icon_8.jpg Human Interleukin-3 Recombinant (CHO cell)
product_icon_7.jpg Rabbit anti IL-3 Polyclonal
product_icon_8.jpg Mouse Interleukin-33 Recombinant
product_icon_8.jpg Human IL-31R beta Recombinant (OSMR)
product_icon_8.jpg Human IL-35 Recombinant
product_icon_8.jpg Human IL-3 receptor Recombinant (CD123)
product_icon_8.jpg Human IL-3RB Recombinant (CD131)
product_icon_8.jpg Mouse IL-31R beta Recombinant (OSMRb)
 
 
 
Item name Size Quantity Price(USD) Remove
checkout
Cart Total $