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Mouse Interleukin-2 Recombinant

IL-2

accession:  P04351
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2500
Domain  :  four helix cytokine core
Gene  :  IL2
Catalog no. :  RKP04351
Source:
Optimized DNA sequence encoding Mouse Interleukin-2 mature chain was expressed in Escherichia Coli.

 
Molecular weight:
Native Mouse Interleukin-2, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 17 kDa.

Recombinant IL-2 is a disulfide-linked monomeric protein consisting of 150 amino acid residue subunits, and migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED50 as determined by the dose dependent stimulation of murine CTLL-2 cells
is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MYSMQLASCV TLTLVLLVNS APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ LLMDLQELLS 

        70         80         90        100        110        120 
RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL RHVLDLTQSK SFQLEDAENF 

       130        140        150        160        170 
ISNIRVTVVK LKGSDNTFEC QFDDESATVV DFLRRWIAFC QSIISTSPQ 
(*)Complete precursor sequence shown, expressed recombinant DNA chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant mouse IL-2 was lyophilized from 0.2 μm filtered PBS solution, pH 7.0.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

IL-2

IL2 is produced mainly by T- cells expressing the surface antigen CD4 following cell activation by mitogens or allogen. Interleukin-2 (IL-2) has multiple, sometimes opposing, functions during an inflammatory response. It is a potent inducer of T- cell proliferation and T- helper 1 (Th1) and Th2 effector T-cell differentiation and provides T cells with a long-lasting competitive advantage resulting in the optimal survival and function of memory cells. In a regulatory role, IL-2 is important for the development, survival, and function of regulatory T cells, it enhances Fas- mediated activation-induced cell death, and it inhibits the development of inflammatory Th17 cells. Thus, in its dual and contrasting functions, IL-2 contributes to both the induction and the termination of inflammatory immune responses.

Related Publications:
vaccination with live leishmania major and cpg dna promotes IL-2 production by dermal dendritic cells and nk cell activation
Clin. Vaccine Immunol., Sep 2009; 10.1128/CVI.00249-09.
erk and not p38 pathway is required for il-12 restoration of t cell IL-2 and ifn- in a rodent model of alcohol intoxication and burn injury
J. Immunol., Sep 2009; 183: 3955 - 3962.
a glucocorticoid amplifies IL-2-induced selective expansion of cd4+cd25+foxp3+ regulatory t cells in vivo and suppresses graft-versus-host disease after allogeneic lymphocyte transplantation
Acta Biochim Biophys Sin, Sep 2009; 41: 781 - 791.
self-peptides prolong survival in murine autoimmunity via reduced IL-2/il-7-mediated stat5 signaling, cd8 coreceptor, and v2 down-regulation
J. Immunol., Sep 2009; 183: 3130 - 3138.
single nucleotide polymorphisms in genes for IL-2, il-6 and tnf- influence on osteolysis and survival of total hip arthroplasty
J Bone Joint Surg Br Proceedings, Sep 2009; 91-B: 473.




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