Description
Accession
Q07325
Source
Optimized DNA sequence encoding Human MIG mature chain was expressed in Escherichia Coli.
Molecular weight
Native human MIG(CXCL9) is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 11 kDa. Recombinant MIG(CXCL9) is a monomeric protein consisting of 103 amino acid residue subunits, migrates as an approximately 11 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to chemoattract human peripheral blood T lymphocytes using a concentration range of.0-80.0 ng/ml.
Protein Sequence
MKKSGVLFLL GIILLVLIGV QGTPVVRKGR CSCISTNQGT IHLQSLKDLK QFAPSPSCEK IEIIATLKNG VQTCLNPDSA DVKELIKKWE KQVSQKKKQK NGKKHQKKKV LKVRKSQRSR QKKTT
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant MIG/CXCL9 was lyophilized from1.0 mg/ml solution inmM NaClmM PB pH7.0.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P49682
Interactor
P51677
Biological Process
Molecular function
