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Human hemopexin Recombinant

Hemopexin

accession:  P02790
   Size A     50ug   $ 430
   Size B      100ug  $800
   Size C      1mg  $ 3700
Domain  :  Hemopexin
Gene  :  HPX
Catalog no. :  RKP02790
Source:
Optimized DNA sequence encoding Human Hemopexin was expressed in  HEK293 cells.

 
Molecular weight:
Recombinant human Hemopexin  is a disulfide-linked monomer protein consisting of 450 amino acid residue subunits, and migrates as an approximately 70kDa protein under reducing conditions in SDS-PAGE.

 
Purity:
>96%, as determined by SDS-PAGE and HPLC

 
Protein Sequence:
        10         20         30         40         50         60 
MARVLGAPVA LGLWSLCWSL AIATPLPPTS AHGNVAEGET KPDPDVTERC SDGWSFDATT 

        70         80         90        100        110        120 
LDDNGTMLFF KGEFVWKSHK WDRELISERW KNFPSPVDAA FRQGHNSVFL IKGDKVWVYP 

       130        140        150        160        170        180 
PEKKEKGYPK LLQDEFPGIP SPLDAAVECH RGECQAEGVL FFQGDREWFW DLATGTMKER 

       190        200        210        220        230        240 
SWPAVGNCSS ALRWLGRYYC FQGNQFLRFD PVRGEVPPRY PRDVRDYFMP CPGRGHGHRN 

       250        260        270        280        290        300 
GTGHGNSTHH GPEYMRCSPH LVLSALTSDN HGATYAFSGT HYWRLDTSRD GWHSWPIAHQ 

       310        320        330        340        350        360 
WPQGPSAVDA AFSWEEKLYL VQGTQVYVFL TKGGYTLVSG YPKRLEKEVG TPHGIILDSV 

       370        380        390        400        410        420 
DAAFICPGSS RLHIMAGRRL WWLDLKSGAQ ATWTELPWPH EKVDGALCME KSLGPNSCSA 

       430        440        450        460 
NGPGLYLIHG PNLYCYSDVE KLNAAKALPQ PQNVTSLLGC THH
(*)Complete precursor sequence shown, expressed chain highlighted 
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant HPX is supplied as 0.2 μm filtered solution in PBS, pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

 
Storage:
This recombinant protein can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months,  without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This  product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

Hemopexin

Hemopexin (HPX) is the plasma protein with the highest binding affinity to heme among known proteins. It is mainly expressed in liver, and belongs to acute phase reactants, the synthesis of which is induced after inflammation. Heme is potentially highly toxic because of its ability to intercalate into lipid membrane and to produce hydroxyl radicals. The binding strength between heme and HPX, and the presence of a specific hemeľ HPX receptor able to catabolize the complex and to induce intracellular antioxidant activities, suggest that hemopexin is the major vehicle for the transportation of heme in the plasma, thus preventing heme-mediated oxidative stress and heme-bound iron loss.

Related Publications:
crystal structure and functional insights of Hemopexin fold protein from lathyrus sativus
Plant Physiology, Feb 2010; 10.1104/pp.109.150680.
mmp20 Hemopexin domain mutation in amelogenesis imperfecta
Journal of Dental Research, Jan 2010; 89: 46 - 50.
identification of specific Hemopexin-like domain residues that facilitate matrix metalloproteinase collagenolytic activity
J. Biol. Chem., Sep 2009; 284: 24017 - 24024.
Hemopexin down-regulates lps-induced proinflammatory cytokines from macrophages
J. Leukoc. Biol., Aug 2009; 86: 229 - 235.
vascular contraction and preeclampsia: downregulation of the angiotensin receptor 1 by Hemopexin in vitro
Hypertension, Jun 2009; 53: 959 - 964.




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