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Mouse FLT3 Ligand Recombinant

FLT3

accession:  P49772
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 3500
Domain  :  four helix cytokine core
Gene  :  FLT3LG
Catalog no. :  RKP49772
Source:
Optimized DNA sequence encoding mouse FLT-3 ligand mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native mouse FLT-3 Ligand, generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated molecular mass of approximately 19 kDa.

Recombinant FLT-3 Ligand is a disulfide-linked monomer protein consisting of  164 amino acid residue subunits, and migrates as an approximately 32 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent proliferation of human AML5 cells was found to be in the range of 10.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MTVLAPAWSP NSSLLLLLLL LSPCLRGTPD CYFSHSPISS NFKVKFRELT DHLLKDYPVT 

        70         80         90        100        110        120 
VAVNLQDEKH CKALWSLFLA QRWIEQLKTV AGSKMQTLLE DVNTEIHFVT SCTFQPLPEC 

       130        140        150        160        170        180 
LRFVQTNISH LLKDTCTQLL ALKPCIGKAC QNFSRCLEVQ CQPDSSTLLP PRSPIALEAT 

       190        200        210        220        230 
ELPEPRPRQL LLLLLLLLPL TLVLLAAAWG LRWQRARRRG ELHPGVPLPS HP
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant FLT-3 Ligand was lyophilized from a 0.2 μm filtered solution with 50mM Tris,100mM NaCl, pH 8.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

FLT3 Ligand

FLT-3 ligand promotes long- term expansion and differentiation of human pro-B-cells in the presence of IL7 or in combination of IL7 and IL3.The human flt-3 ligand also stimulates the proliferation of cells expressing murine flt-3 receptors.n combination with SCF and IL3 flt-3 ligand can cause expansion of cells with the marker spectrum CD34(+) CD38(-). Alone, flt- 3 ligand supports the survival of precursors in the lineage of blood-forming cells such as CFU-GM, CFU- GEMM, and the very primitive high proliferative potential colony-forming cells, HPP- CFC. flt-3 ligand only has marginal effects on progenitors for erythroid cells and megakaryocytes.

Related Publications:
tumor vaccines expressing FLT3 Ligand synergize with ctla-4 blockade to reject preimplanted tumors
Cancer Res., Sep 2009; 10.1158/0008-5472.CAN-08-3289.
the concerted action of gm-csf and flt3-ligand on in vivo dendritic cell homeostasis
Blood, Jul 2009; 114: 835 - 843.
ectodomain shedding of FLT3 Ligand is mediated by tnf- converting enzyme
J. Immunol., Jun 2009; 182: 7408 - 7414.
transplanted cd34+ cells stimulated with FLT3 Ligand and granulocyte-colony stimulating factor display an impaired early reconstitution capacity
Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 4591.
hematopoietic stem - and progenitor cells are differentially mobilized in response to flt3-ligand
Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 4782.




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