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Mouse Epidermal Growth Factor Recombinant

EGF

accession:  P01132
   Size A     100ug   $ 70
   Size B      500ug  $160
   Size C      1mg  $ 230
Domain  :  EGF
Gene  :  EGF
Catalog no. :  RKP01132
Source:
Optimized DNA sequence encoding Mouse EGF mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Recombinant Mouse EGF, generated by the proteolytic removal of the signal peptide
and propeptide, is a disulfide-linked monomer  protein.

Recombinant Mouse EGF consists of 54 amino acids and migrates as an approximately 6.2 kDa protein under reducing conditions.


 
Purity:
>97%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

 
Protein Sequence:
 
 
       970        980        990       1000       1010       1020 
STAPSLLGED GHHLDRNSYP GCPSSYDGYC LNGGVCMHIE SLDSYTCNCV IGYSGDRCQT 

      1030       1040       1050       1060       1070       1080 
RDLRWWELRH AGYGQKHDIM VVAVCMVALV LLLLLGMWGT YYYRTRKQLS NPPKNPCDEP 


(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Mouse EGF was lyophilized from a 0.2 μm filtered solution in PBS, pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

EGF

Epidermal growth factor (EGF) is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites. EGF receptors are expressed in almost all types of tissues . Parietal endoderm, mature skeletal muscles, and hematopoietic tissues do not express the receptor. The EGF receptor, designated HER1 , is a 170 kDa transmembrane glycoprotein with a length of 1186 amino acids. It is identical with a previously described glycoprotein called SA-7 (species antigen 7). The extracellular receptor domain has a length of 621 amino acids, including 11 glycosylated asparagine residues and 51 cysteine residues. This domain contains the EGF binding site and also binds mammalian TGF-alpha.

Related Publications:
thrombin induces EGF receptor expression and cell proliferation via a pkc()/c-src-dependent pathway in vascular smooth muscle cells
Arterioscler Thromb Vasc Biol, Oct 2009; 29: 1594 - 1601.
dependence of EGF-induced increases in corneal epithelial proliferation and migration on gsk-3 inactivation
Invest. Ophthalmol. Vis. Sci., Oct 2009; 50: 4828 - 4835.
internalization and intracellular sorting of the EGF receptor: a model for understanding the mechanisms of receptor trafficking
J. Cell Sci., Oct 2009; 122: 3433 - 3439.
angiotensin ii maintains cerebral vascular relaxation via EGF receptor transactivation and erk1/2
Am J Physiol Heart Circ Physiol, Oct 2009; 297: H1296 - H1303.
g protein-coupled receptor 30 expression is up-regulated by EGF and tgf in estrogen receptor -positive cancer cells
Mol. Endocrinol., Sep 2009; 10.1210/me.2009-0120.




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