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Mouse SDF-1 alpha Recombinant

SDF-1

accession:  P40224
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 2700
Domain  :  IL8
Gene  :  CXCL12
Catalog no. :  RKP40224
Source:
Optimized DNA sequence encoding Mouse SDF-1 alpha mature chain was expressed in Escherichia Coli.

 
Molecular weight:
Native Mouse Stromal Cell-Derived Factor-1 alpha alpha, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately 8 kDa.

Recombinant SDF-1 alpha is a disulfide-linked monomer protein consisting of 69 amino acid residue subunits, migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

 

Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentration range of 5-40 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MDAKVVAVLA LVLAALCISD GKPVSLSYRC PCRFFESHIA RANVKHLKIL NTPNCALQIV 

        70         80         90 
ARLKNNNRQV CIDPKLKWIQ EYLEKALNK 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant SDF-1 alpha was lyophilized from a 0.2 μm filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween80, 5 mM Glutamic acid, pH 4.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

SDF-1 alpha

SDF-1-alpha and SDF-1-beta are small cytokines belonging to the CXC- Chemokines. SDF-1 is identical with a chemokine reported to function as a pre-B-cell growth factor in the presence of IL7 and isolated originally from a murine bone marrow stromal cell line.Human SDF-1-alpha and SDF-1-beta are encoded by a single gene and arise by alternative splicing. SDF-1 acts on lymphocytes and monocytes but not neutrophils in vitro and is a highly potent chemoattractant for mononuclear cells in vivo. In addition. SDF-1 also induces intracellular actin polymerization in lymphocytes. SDF acts as a chemoattractant for human hematopoietic progenitor cells expressing CD34 giving rise to mixed types of progenitors, and more primitive types. The chemotactic response is inhibited by pertussis toxin. Chemotaxis of CD34(+) cells in response to SDF is increased by IL3 in vitro. SDF has been shown also to induce a transient elevation of cytoplasmic calcium in these cells.

Related Publications:
inhibition of the sdf-1-cxcr4 axis by the cxcr4 antagonist amd3100 suppresses the migration of cultured cells from atl patients and murine lymphoblastoid cells from htlv-i tax transgenic mice
Blood, Aug 2009; 10.1182/blood-2008-11-189308.
sdf-1 stimulates jnk3 activity via enos-dependent nitrosylation of mkp7 to enhance endothelial migration
PNAS, Apr 2009; 106: 5675 - 5680.
stem cell homing and angiomyogenesis in transplanted hearts are enhanced by combined intramyocardial sdf-1 delivery and endogenous cytokine signaling
Am J Physiol Heart Circ Physiol, Apr 2009; 296: H976 - H986.
stromal cell-derived factor-1 (sdf-1/cxcl12)-enhanced angiogenesis of human basal cell carcinoma cells involves erk1/2–nf-b/interleukin-6 pathway
Carcinogenesis, Feb 2009; 30: 205 - 213.
igf-1–overexpressing mesenchymal stem cells accelerate bone marrow stem cell mobilization via paracrine activation of sdf-1/cxcr4 signaling to promote myocardial repair
Circ. Res., Nov 2008; 103: 1300 - 1308.




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